摘要
组织培养和转基因技术是当今植物研究的热点。近年来,关于牡丹再生体系的建立仍存在再生体系不完善、再生效率低、生根难、褐化率高等问题。而转基因研究是以成熟的再生体系的建立为基础,因此,关于牡丹的转基因研究更为匮乏。为了建立高效的再生体系和遗传转化体系,本研究综述了国内外近年来关于牡丹组织培养的方法,分析了不同方法的诱导效率,同时提出了防止褐化,提高生根率的对策。
Tissue culture and transgenic technology are central issues of modern biotechnology.In recent years,there are problems in peony regeneration system construction,such as incomplete regeneration system,low regeneration efficiency,difficult rooting and high browning rate.Since the transgenic research is based on the establishment of an efficient regeneration system,so it is still scarce concerning peony.In order to establish an efficient regeneration and genetic transformation system for peony,we summarize the tissue culture of peony at home and abroad in recent years and analyze the induction efficiency,at the same time,put forward advices on preventing browning and improving the rooting rate.
作者
杨贞
张永春
杨柳燕
陈敏敏
蔡友铭
Yang Zhen;Zhang Yongchun;Yang Liuyan;Chen Minmin;Cai Youming(Institute of Forestry Pomology,Shanghai Academy of Agricultural Sciences/Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201403)
出处
《中国农学通报》
2019年第11期56-60,共5页
Chinese Agricultural Science Bulletin
基金
市农委产业体系建设专项"上海市花卉产业技术体系建设"(沪农科产字(2019)第8号)
市科委研发平台建设项目"上海市植物种苗组培专业技术服务平台"(18DZ2291400)
