西红花组织培养研究进展

Tissue Culture of Crocus Sativus L.:Research Progress

为了研究人工繁育西红花种球的途径,笔者综述了国内外30多年以来的西红花组织培养研究进展,发现利用不同的外植体都有诱导形成小球茎或完整植株的报道,但是其诱导周期长、诱导率较低,未见进行大规模产业化生产的报道。因此,今后应该着重于研究如何缩短诱导周期,提高诱导率、增殖率,加快大球茎培育进程,并进行花器官和子房的组织培养研究,提高柱头状物诱导率,加强细胞大规模培养技术研究,推动次生代谢产物生产的规模开发。笔者就西红花组织培养的研究成果进行了归纳与综述,并对西红花组培进展进行了展望,以期为中国西红花种球规模化和产业化生产提供参考。

The paper aims to study the ways of artificial breeding of saffron,we review the research progress on tissue culture of saffron in China and abroad for more than 30 years.It is found that the formation of small bulbs or intact plant could be induced by using different explants,but the induction period is long and the induction rate is low,so no large-scale industrial production is reported.Therefore,in the future,we should focus on how to shorten the induction period,increase the rate of induction and proliferation,and accelerate the process of the large bulb cultivation.In addition,the research should be carried on the tissue culture of flower organs and ovary,and the improvement of induction rate of stigmaculture should also be strengthened to promote the scale development of the production of secondary metabolites.The paper summarizes the research progress on achievements of tissue culture of saffron,and discusses its future prospects in order to provide reference for large scale propagation and industrial production of saffron in China.