Wnt7a和孕酮受体在人工受精奶牛受孕前后表达规律研究

Wnt7a and Progesterone Receptors: The Expression in Artificially Fertilized Cows Before and After Conception

旨在筛选奶牛早期妊娠诊断标志因子,提高奶牛养殖经济效益。在北京某牛场选10头荷斯坦奶牛,在输精后第1、4、7、10、13、16、19天取血,通过荧光定量PCR和western blotting方法分析Wnt7a和PR的表达趋势和变化规律。通过直检对10头荷斯坦奶牛进行怀孕检测,其中有7头受孕3头未受孕。通过检测受孕和未孕的奶牛血液中Wnt7a基因和PR基因的表达量,发现Wnt7a基因在受孕奶牛血液中的表达量均高于未孕奶牛,特别是输精后第7天受孕奶牛的表达量显著增高,Wnt7a的蛋白质水平表达呈现出类似趋势;PR基因在全部10头奶牛中呈现出从输精后第一天表达开始降低,并一直维持一个很低的表达状态。Wnt7a具有作为奶牛早期妊娠诊断标志因子的潜质,其优势在于将检测窗口提前到输精后的第7天;PR不具有作为标志因子的优势。通过探究影响早期妊娠的相关因子变化规律,可以为孕检的时间窗口提前到早期妊娠阶段提供理论依据。

This study aims at screening the early pregnancy diagnosis marker of dairy cows and improving the economic benefits of dairy farming.Ten Holstein cows were selected from some cattle farms in Beijing.Blood was taken on the 1st,4th,7 th,10th,13th,16 th,and 19th day after insemination,the expression trends and changes of Wnt7 a and PR were analyzed by fluorescence quantitative PCR and western blotting.The pregnancy test of 10 Holstein cows was conducted with the direct inspection,7 of them were conceived and 3 were unpregnancy.By detecting the expression levels of Wnt7 a and PR genes in the blood of pregnant and non-pregnant cows,it was found that the expression level of Wnt7 a gene in the blood of pregnant cows was higher than that of nonpregnant cows.In particular,the expression level of Wnt7 a gene in pregnant cows increased significantly on the 7 thday after insemination,the level expression of Wnt7 a protein showed the similar trend.The PR gene showed a decreased-expression trend on the 1 stday after insemination in all 10 cows and it maintained a very low expression state.Wnt7 a has the potential as a diagnostic marker for early pregnancy in dairy cows,which has the advantage of advancing the detection window to the 7 thday after insemination;PR does not have the advantage as a marker factor.By exploring the changes of the relative factors affecting early pregnancy,it can provide a theoretical basis for the time window of pregnancy test advancing to the early pregnancy stage.