白木香的组织培养快速繁殖研究

Rapid Propagation of Aquilaria sinensis by Tissue Culture Technology

本研究利用组织培养技术,以白木香(Aquilaria sinensis)种子为材料,建立快速繁殖白木香的方法体系。首先进行种子消毒,播种到MS培养基上,然后将白木香无菌幼苗切成带1个腋芽的茎段,诱导白木香丛生芽;剪下生长健壮的单个芽苗,诱导生根。白木香腋芽诱导分化的最佳培养基为MS+1 mg/L6-BA+0.2 mg/L NAA+0.2 mg/L IBA+30 g/L蔗糖+5 g/L植物凝胶,白木香组培苗生根的最佳培养基为MS+30 g/L蔗糖+5 g/L植物凝胶。白木香组织培养快速繁殖技术将应用于白木香特异种质的快速大量扩繁中。另外,由于白木香种子随采随种、不易保存的特性,组培快繁技术也可随时为白木香结香机理研究提供遗传背景相对一致的大量新鲜幼嫩的组织材料。

In this study,tissue culture technology was used to establish a rapid propagation system of Aquilaria sinensis with the seeds.Firstly,seeds were sterilized and germinated on MS medium.Then,the sterile seedlings were cut into segments with one potential lateral bud to be induced to grow into cluster buds.Finally,the single growing-up plantlets were cut off for rooting.The best medium for differentiation was MS+1 mg/L6-BA+0.2 mg/L NAA+0.2 mg/L IBA+30 g/L sucrose+5 g/L plant gel,and the best medium for rooting of tissue cultured plantlets was MS+30 g/L sucrose+5 g/L plant gel.The establishment of rapid propagation system for A.sinensis will be applied to the rapid massive propagation of the special germplasms of A.sinensis.In addition,due to the hard preservation of seeds of A.sinensis,the rapid propagation technology can also provide a large number of fresh and tender tissue materials with relatively identical genetic background for the study of agarwood formation mechanism.