蒙药槟榔十三味丸(高尤-13)对慢性应激抑郁模型大鼠肝肾毒性的影响

Effect of Mongolian Betel Shisanwei Ingredients Pill (Gao-You-13) on Liver and Kidney Toxicity of Chronic Stress Depression Model Rats

为观察槟榔十三味丸(高尤-13)对慢性应激抑郁模型大鼠肝肾毒性的影响,随机将48只Wistar雄性大鼠根据蔗糖水消耗量分为6组:槟榔十三味丸低、中、高剂量组(0.25,0.5,1.0 g·kg^(-1)),氟西汀组(3.3 mg·kg-1),模型组和正常对照组,每组8只.除正常对照组外,其余大鼠均采用慢性轻度不可预见性应激结合孤养方法制备抑郁模型,造模同时灌胃给药,每日1次,连续给药28 d后,检测大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素(UREA)、肌酐(CREA)含量,HE染色法观察肝肾组织形态学变化.结果显示:模型组大鼠较正常对照组血清ALT含量显著升高(P<0.01),肝细胞轻度肿胀、变性,肝窦缩窄,细胞界限不清(P<0.05).槟榔十三味丸低、中、高剂量组较模型组血清ALT含量显著降低(P<0.01或P<0.05),给药各组与氟西汀组大鼠的肝脏未见与药物相关的毒性病变(P>0.05).各组大鼠血清AST、UREA、CREA含量无显著差异,肾脏病理变化不显著(P>0.05).说明槟榔十三味丸对慢性应激抑郁模型大鼠无肝肾毒性,并具有肝损伤修复作用.

In order to observe the effects of Betel Shisanwei ingredients pill( Gao-You-13) on the liver and kidney toxicity of chronic stress depression model rats,48 male Wistar rats were randomly divided into six groups according to the sugar consumption test( 8 rats in each group) : low,medium and high dose group( 0. 25,0. 5,1. 0 g·kg^(-1)) of Gao-You-13 group,fluoxetine group( 3. 3 mg·kg^(-1)),model group and normal control. Each group was treated with the chronic unpredictable mild stress stimulation under separation except the normal control group. Meanwhile,the rats were given the drugs once daily for 28 days. Then the contents of ALT,AST,UREA and CREA in rats' serum were tested. The liver and kidney tissue morphological changes were observed by HE staining. The results showed that compared with the normal control,ALT levels in model group increased significantly( P < 0. 01) and most liver cells appeared mild swelling and degeneration,hepatic sinus narrowed,cell boundaries were unclear( P < 0. 05); while ALT levels in Gao-You-13 group decreased significantly compared with the model group( P < 0. 01 or P < 0. 05). Drug related toxicity lesions did not appear in Gao-You-13 and fluoxetine treatment group( P >0. 05). AST,UREA,CREA contents and kidney pathological changes did not show significant differences in each group( P >0. 05). It was confirmed that Gao-You-13 could repair liver damages without liver and kidney toxicity for the chronic stress depression model rats.