达托霉素发酵工艺条件响应面优化研究

Study on response surface methodology for doxymycin fermentation

为优化达托霉素发酵条件,通过单因素实验和响应面方法研究了达托霉素发酵条件温度、pH值、接种量、通风量和搅拌转速.结果表明:优化后的发酵工艺条件为温度27.74℃、pH值8.36、接种量8.48%、通风量6.13 L/min、搅拌转速242 r/min,在此条件下达托霉素的产量可达880.48 mg/L,比最新报道的达托霉素发酵效价提高了132%.本方法大幅度提高了达托霉素产量,对达托霉素工业化生产提供了可靠的研究基础.

In order to optimize the fermentation conditions of doxymycin, the fermentation conditions of doxymycin, temperature, pH, inoculation, ventilation and stirring speed, were studied by single factor test and response surface method. The results showed that the optimized fermentation process conditions were 27.74 ℃, pH 8.36, inoculation 8.48%, ventilation 6.13 L/min, and stirring speed 242 r/min. Under this condition, the yield of doxymycin can reach 880.48 mg/L, which was 132% higher than the newly reported fermentative effective potency of doxymycin. This study has greatly increased the production efficiency of doxymycin and provided a reliable research basis for the industrial production of doxymycin.