猪卵母细胞体外成熟和孤雌激活效率影响因素分析

Effects of Age of Donors and Conditions of Preserving Ovaries on Porcine Oocytes Maturation in vitro and Efficiency of Parthenogenetic Activation

研究了月龄和卵巢采集后的保存条件对猪卵母细胞体外成熟和孤雌激活效率的影响 ,以确立猪卵母细胞体外成熟的最佳条件。试验包括 :(1)卵巢保存的生理盐水温度 (2 2、30、37、38.5、4 0℃ )对猪卵母细胞体外成熟和发育潜力的影响。 (2 )卵巢保存时间对猪卵母细胞体外成熟和后期发育的影响。 (3)初情期前后母猪卵母细胞对体外成熟和发育潜力的影响。结果表明 ,(1) 38.5℃保存的卵巢卵母细胞激活后的卵裂率 (79.6 4 % )和囊胚率(18.11% ) ,37℃的卵裂率 (76 .18% )和囊胚率 (33.82 % )相比 ,差异不显著 (P >0 .0 5 )。当温度达到 4 0℃时 ,卵裂率(2 1.6 8% )和囊胚率 (0 )与 37℃相比差异极显著 (P <0 .0 1)。 30℃与 37℃相比 ,卵裂率和囊胚率之间差异不明显 (P>0 .0 5 )。而当温度降低到 2 2℃时 ,卵裂率和囊胚率明显降低 (P <0 .0 5 )。 (2 )猪卵巢在体外保存 2和 6h对卵母细胞激活后的卵裂率 (6 8.86 %VS 6 8.74 % )和囊胚率 (16 .12 %VS 16 .0 9% )无显著影响 (P >0 .0 5 )。 (3)初情期前、后母猪卵母细胞激活后的卵裂率没有显著差异 (72 .90 %± 7.0 4 %VS 77.35 %± 8.2 9%P >0 .0 5 ) ,但初情期后母猪卵母细胞激活后的囊胚发育率显著高于初情期前 (2 2 .5 2 %± 7.4 8%VS 36 .6 2 %± 10 .2 5 % 。

Effects of different ages of donors and different conditions of preserving ovaries on porcine oocytes maturation in vitro and efficiency of parthenogenetic activation were studied. The experiments included that: (1) The effects of different temperatures (22, 30, 37, 38.5, 40℃) of preserving ovaries on maturation in vitro and developmental potential of porcine oocytes. (2) The effects of periods of preserving ovaries on in vitro maturation and in vitro development of porcine oocytes. (3) The effects of different ages of donors on in vitro maturation and developmental potential of porcine oocytes . The results showed that: (1) There were no statistical differences (P>0.05) of the parthenogenetic cleavage rate (79.64% VS 76.18%) and blastocyst rate (18.11% VS 33.82%) between oocytes from ovaries preserved in 38.5℃ and those preserved in 37℃ saline. However, when the preserving temperature was increased to 40℃, the cleavage rate (19.37%) and the blastocyst rate (0) were greatly significant lower than those at 37℃ (P<0.01). The cleavage rate (68.60% vs 64.68%) and blastocyst rate (18.30% VS 20.90%) were not different between 30℃ and 37℃(P>0.05). When the preserving temperature decreased to 22℃, the rate of cleavage was not different, but the rate of blastocyst was significantly lower than that of 37℃. (2) The rate of cleavage and blastocyst of the porcine oocytes of ovaries preserved in saline at 37℃ collected at 2 or 6 h after cultured in vitro and activated were not different (P>0.05). (3) The cleavage rates of oocytes from gilts or sows after in vitro maturation and activation were not different between gilt and sows, but the rate of blastocyst of the sow group was significant higher than that of gilt group (P<0.05). The blastocyst cell number of sows and gilt showed no difference (P>0.05).