环丙沙星铽荧光配合物与DNA的相互作用及染色新方法

Interaction Between DNA and Terbium(Ⅲ)-Ciprofloxacin Fluorescence Complex and New Tissue Staining Method

合成了环丙沙星铽荧光配合物 ,并研究其与 DNA分子的相互作用 .对比 DNA分子吸附配合物分子前后的显微红外及荧光光谱发现 ,以环丙沙星分子为配体的稀土配合物同 DNA间存在相互作用 ,这种作用诱导了 DNA二级结构的构象变化 .基于这种相互作用 ,研究了环丙沙星铽配合物对口腔粘膜组织切片的荧光染色 ,得到了同经典的苏木精

A new lanthanide complex that gives off green fluorescence upon exciting by UV radiation was synthesized by using terbium chloride and ciprofloxacin. The complex binds firmly on DNA and induces conformation changes of DNA based on the analysis of FTIR and fluorescence spectra. Strong fluorescence from terbium ions can be observed from the resultant composite under UV light. Thus, it is possible that the terbium ciprofloxacin complex can be developed as a fluorescent dye in biological and pathological studies. The results indicate that the complex is concentrated on the nuclei regions in the sections of oral tissues and the nuclei are highlighted under fluorescent microscope.