HPV16型-E6、E7在食管鳞癌组织与非癌组织中的表达

Expression of HPV16-E6 and E7 Oncoproteins in Squamous Cell Carcinoma Tissues of Esophageal Cancer and Non-cancer Tissues

背景与目的:越来越多的证据表明高危型人乳头状瘤病毒(humanpapillomavirus,HPV),特别是HPV16型与多种肿瘤发生、发展密切相关。本研究旨在分析人乳头状瘤病毒HPV16型E6、E7在食管正常组织、不典型增生组织和癌组织中的表达,以探讨HPV16型在食管鳞癌发生、发展中的生物学意义。方法:采用PicTureTM免疫组织化学方法对70例食管癌切除标本上、下切缘的正常粘膜上皮组织、43例不典型增生组织以及18例癌组织中的HPV16型E6、E7蛋白表达情况进行研究。结果:E6蛋白阳性率在正常食管粘膜上皮组织中为59.3%,在上皮不典型增生组织中为88.4%,在癌组织中为83.3%;E7蛋白在上述3种组织中的阳性率分别为62.1%、90.7%和88.9%。与正常食管粘膜上皮相比,上皮不典型增生组织和癌组织中E6、E7蛋白表达均有显著性差异(P<0.05)。HPV16型E6、E7蛋白在正常食管粘膜组织中同时表达即同步率为25.7%,而在上皮不典型增生组织和癌组织中二者同步率分别为88.3%和83.3%。结论:HPV16型E6、E7蛋白与食管鳞癌发生、发展密切相关,二者协同作用可能是食管鳞癌发生、发展的重要因素之一。

BACKGROUND &OBJECTIVE: Evidences indicate that high risk type human papillomavirus (HPV) are closely associated with the carcinogenesis, progression and transformation of several kinds of human tumors. This study was designed to determine the expression of HPV16 E6 and E7 oncoproteins in normal tissues, dysplasia tissues, and carcinoma tissues of patients with esophageal cancer and to investigate the biological significance of high risk type HPV in the esophageal squamous cell carcinogenesis. METHODS: HPV16 E6 and E7 oncoproteins were determined using immunohistochemical staining in normal mucosa tissues (70 cases), dysplasia tissues (43 cases), and carcinoma tissues (18 cases). RESULTS: The positive rates of HPV16 E6 in the tissues of normal mucosa, dysplasia, and carcinoma of esophagus patients were 59.3%,88.4%,and 83.3%,respectively; the positive rates of HPV16 E7 protein were 62.1%, 90.7%, and 88.9%, respectively. The positive rates of HPV16 E6 and E7 in dysplasia and carcinoma of esophagus were significantly higher than those in normal mucosa (P< 0.05). Double expression of HPV16 E6 and E7 in normal mucosa was 25.7%, while in dysplasia and carcinoma were 88.3%and 83.3%,respectively. CONCLUSION: HPV16 E6 and E7 are highly associated with esophageal squamous cell carcinogenesis. And cooperation of HPV16 E6 and E7 may play an important role in genesis of esophageal squamous carcinoma.