食管癌组织及其区域淋巴结中树突细胞CD1a、CD80、CD86的表达

Expression of CD1a, CD80, CD86 in Dendritic Cell of Tumor Tissue and Regional Lymph Node in Esophageal Carcinoma

背景与目的:树突细胞(dendriticcell,DC)是重要的抗原提呈细胞,参与抗肿瘤免疫。本研究通过检测人食管癌组织及其区域淋巴结中树突细胞CD1a(特异性标志)、CD80、CD86(共刺激分子)的表达,探讨食管癌发生及其免疫逃逸的机制。方法:采用流式细胞术检测58例食管癌病例(Ⅰ-Ⅱ期27例,Ⅲ-Ⅳ期31例;G1-237例,G321例;鳞癌49例,腺癌9例;有淋巴结癌转移者37例,无淋巴结癌转移者21例)的癌组织及癌旁组织、11例食管炎性组织、12例正常食管组织、31枚有癌转移的区域淋巴结、34枚无癌转移的区域淋巴结中树突细胞CD1a及CD80、CD86的表达。结果:(1)树突细胞CD1a、CD80、CD86的表达在食管癌组织中(6.18±1.47,5.37±1.13,37.35±7.42)明显低于正常食管组织(10.28±2.11,9.67±1.94,48.76±10.23)、食管炎性组织(11.89±2.65,10.46±1.79,51.55±10.60)及癌旁组织(11.79±2.41,10.49±1.89,9.78±12.31)中的表达(P<0.01);(2)树突细胞CD1a、CD80、CD86的表达在食管癌患者有癌转移的区域淋巴结中的表达(8.34±1.66,6.78±1.42,41.70±8.71)明显低于无癌转移的淋巴结中的表达(12.23±2.14,10.82±2.11,59.63±12.52)(P<0.01);(3)癌旁组织及食管炎性组织中树突细胞CD1a、CD80、CD86的表达与正常食管组织中的表达的差异无统计学?

BACKGROUND &OBJECTIVE: Dendritic cells are the important antigen presenting cell, and take part in the anti tumor immunoreaction. This study was designed to investigate the expression of CD1a (specific marker) and CD80, CD86 (costimulating molecules) in dendritic cell of tumor tissue and regional lymph node of esophageal carcinoma and to investigate the mechanisms by which tumors escape immune recognition. METHODS: The expression of CD1a,CD80,CD86 in dendritic cells of tumor tissues and paratumor tissues from 58 patients with esophageal carcinoma (clinical period Ⅰ Ⅱ:27 cases,Ⅲ Ⅳ: 31 cases;differentiation G1-2:37 cases,G3:21 cases;squamous cancer:49 cases,adenoid cancer:9 cases;lymph node metastases present:37 cases,lymph node metastases absent:21 cases),inflammatory tissues from 11 patients with esophagitis,12 normal esophageal tissues (as control),31 regional lymph nodes with tumor and 34 regional lymph nodes without tumor were determined using flow cytometry. RESULTS: (1) The expression rates of CD1a, CD80, CD86 in tumor tissues (6.18±1.47, 5.37±1.13, 37.35±7.42) were less than those in normal tissues (10.28±2.11, 9.67±1.94, 48.76±10.23), the inflammatory tissues (11.89±2.65, 10.46±1.79, 51.55±10.60), and the paratumor tissue (11.79±2.41, 10.49±1.89, 9.78±12.31)(P< 0.01). (2) The expression rates of CD1a,CD80,CD86 in regional lymph node with tumor (8.34±1.66,6.78±1.42,41.70±8.71) were less than those in the lymph node without tumor (12.23±2.14, 10.82±2.11, 59.63±12.52)(P< 0.01). (3) There was no significant difference in the expression rates of CD1a, CD80, CD86 between paratumor tissue, inflammatory tissue and normal esophageal tissues (P >0.05). (4) The expression of CD1a,CD80,CD86 were not associated with the clinical period, pathological type, and the lymph node metastases (P >0.05); but were associated with pathological differentiation (grade1 2 were higher than grade 3)(P< 0.01). CONCLUSION: (1) The number of dendritic cell of CD1a (+) decreases in tumor tissue and regional lymph node with tumor in esophageal carcinoma and the expression of CD80, CD86 are down regulated. (2) The lower the tumor differentiation grade, the less the number of CD1a(+)dendritic cell in tumor tissue and regional lymph node and the lower the expression of CD80, CD86 in tumor tissue.