非小细胞肺癌中MAGE-A1的表达与Ki-67表达及TIL反应的相关性

Relationship of MAGE-A1 Expression with Ki-67 and Tumor-Infiltrating Lymphocyte Response in Non-Small Cell Lung Carcinoma

背景与目的:已有研究指出肿瘤特异性抗原———MAGE(melanomaantigen)家族中某些成员的表达可能与增殖旺盛的幼稚细胞关系密切,但鲜见与肿瘤细胞的增殖及宿主局部免疫反应关系的报道。本研究旨在探讨非小细胞肺癌(non-smallcelllungcarcinoma,NSCLC)中MAGE-A1表达与Ki-67表达、肿瘤浸润淋巴细胞(tumor-infiltratinglymphocytes,TIL)反应以及组织学分级和病理类型的相关性。方法:采用免疫组化SP法检测甲醛固定、石蜡包埋的30例NSCLC标本中MAGE-A1、Ki-67表达水平及TIL反应状况。结果:MAGE-A1阳性率为80.00%(24/30),高表达和低表达分别为58.33%(14/24)和41.67%(10/24)。Ki-67阳性率为93.33%(28/30),高表达和低表达分别为57.14%(16/28)和42.86%(12/28)。TIL反应阳性22例。MAGE-A1阳性率的高低与Ki-67阳性表达的高低及肿瘤TIL反应强度之间均呈正相关(rs分别为0.578和0.505,P<0.005),其表达状况在不同组织学分级和病理类型之间的差异无显著性(P>0.05)。结论:NSCLC中MAGE-A1阳性表达的肿瘤细胞具有高增殖活性;同时MAGE-A1表达上调显示肿瘤细胞抗原性增强,引起局部TI反应相应增强。提示对此类患者,有望利用MAGE-A1作为免疫治疗的攻击靶点。

BACKGROUND &OBJECTIVE: Recent studies revealed a possible close association between the expression of some members of tumor specific antigen MAGE (melanoma antigen) family and actively proliferated infantile cells. But the correlation of MAGE A1 expression with proliferation of tumor cells and immune response at host local site has not been reported to date. Our study was to investigate the expression of MAGE A1 in non small cell lung carcinoma (NSCLC), and its relationship with Ki 67 expression, tumor infiltrating lymphocyte (TIL) response, histologic grade, and pathological type. METHODS: Thirty NSCLC samples in formalin fixed, paraffin embedded sections were examined for MAGE A1, Ki 67 and TIL response using SP immunohistochemical technique. RESULTS: The positive expression rate of MAGE A1 was 80.00%(24/30) with high expression rate of 58.33%(14/24) and low expression rate of 41.67%(10/24).The positive expression rate of Ki 67 was 93.33%(28/30) with high expression rate of 57.14%(16/28) and low expression rate of 42.86%(12/28). TIL response was observed in 22 patients. There was a significant relationship between MAGE A1 positive expression and Ki 67 positive expression (rs=0.578, P< 0.005), as well as between MAGE A1 positive expression and TIL response (rs=0.505, P< 0.005). However, MAGE A1 expression was not significantly correlated with histologic grade and pathological type (P >0.05). CONCLUSION: The NSCLC cells with MAGE A1 positive expression possess high proliferation activity; meanwhile, the up regulation of MAGE A1 indicates the increase of antigen in tumor cells and the increase of local TIL response, indicating that MAGE A1 may have potential to be used as a target for immunotherapy in NSCLC patient.