端粒酶核酶抑制肿瘤细胞生长的研究

On Telomerase Ribozyme Retard the Growth of Tumor Cells

已证明有84．7％的人类恶性肿瘤组织细胞中端粒酶表达异常增高、端粒酶的作用是合成染色体末端端粒重复序列维持染色体稳定及细胞永生化的关键因素．因此，端粒酶在肿瘤发生发展中起重要作用，是目前最广泛的肿瘤标志物．核酶是一种具有内切酶活性的RNA分子,只要满足一定的空间结构就能定点切割RNA底物、切割端粒酶的核酶(简称为端粒酶核酶)主要针对端粒酶的两个亚单位hTR和hTERT抑制两基因的表达，降低端粒酶的活性,抑制肿瘤细胞的生长．

It has proved that telomerase express increased abnormally in (84.7%) human tumor tissues and cells. The function of telomerase is that it can maintain the stability of cromosome by synthesization telomere repeat sequence. Telomerase is the key factor for cell immortallizaiton, so telomerase is the widest marker for tumor. Ribozymes are small catalytic RNA which could cleave RNA substrate orientationally and block its expression. Telomerase ribozymes target mainly the hTR subset and hTERT subset to inhibit their expression and decrease the activity of telomerase so that tumor cell can be proliferated slowly.