赖草属植物醇溶蛋白的遗传多态性

Genetic Polymorphism of Gliadin in Leymus

用酸性聚丙烯酰胺凝胶电泳 (A PAGE)对小麦族赖草属 2 0种和 1亚种的 45份材料进行醇溶蛋白遗传分析 ,结果表明 :(1 ) 4 5份材料共出现 43种不同的醇溶蛋白图谱 ,从中分离出的 38条带纹多态性高达 1 0 0 % ;(2 )赖草属植物具有丰富的醇溶蛋白遗传多态性 ,其种间和种内不同居群间均存在明显的醇溶蛋白遗传变异 ,其种间变异大于种内变异 ,醇溶蛋白图谱可以作为鉴定赖草属植物的指纹图谱 ;(3)醇溶蛋白图谱的聚类结果与形态学结果基本一致 ,表明醇溶蛋白资料可以运用于赖草属植物种间。

The gliadin genetic polymorphism among 45 accessions of 20 species and 1 subspecies in Leymus Hochst.were analyzed by using acid polyacrylamide gel electrophoresis (A-PAGE).The results showed as follows:(1) 43 types of gliadin patterns were obtained from the tested materials and 41 materials had their unique patterns respectively.A total of 38 gliadin bands were separated by electrophoresis,of all showing polymorphism,which amounts to 100%.(2) Distinct gliadin genetic variations were presented among the populations.The gliadin genetic variations among different species were more abundant than that of different accessions,and it revealed that the gliadin patterns can be used as a fingerprint to survey different materials in Leymus Hochst.(3) The relationships among species in Leymus Hochst.according to gliadin data are basically comparable with that obtained from morphological data,so that the gliadin patterns data can be used to investigate genetic variation and biosystematic relationships among species in Leymus Hochst.