华木莲的遗传多样性研究

Study on Genetic Diversity of Manglietia decidua (Magnoliaceae)

利用ISSR标记对中国特有植物华木莲 (ManglietiadeciduaQ .Y .Zheng)的遗传多样性进行了研究 ,从 90个引物中筛选出 1 0个引物用于正式扩增 ,共扩增出 81条DNA带 ,其中多态性DNA带 1 4条 ,占 1 7 2 8% ,平均每个引物扩增的DNA带 8 1条 ,多态性条带 1 4条。结果表明 :华木莲的遗传多样性水平较低 (种水平的遗传多样性HT 为 0 0 649,居群水平的遗传多样性HS 为 0 0 51 5,多态位点百分率P为 1 7 2 8% )。与物种的遗传多样性平均水平、同科的其它种类以及其它特有植物相比 。

Genetic diversity of Manglietia decidua Q.Y.Zheng was investigated using inter-simple sequence repeat markers (ISSR).Ten primers were screened from 90 primers.ISSR amplification generated multiple banding profiles with the 10 primers from all DNA samples,with an average of 8.1 fragments per primer.There were 14 polymorphic loci in the total fragments.Compared with other plants,the genetic diversity of M.decidua is very low.