长链非编码RNA GAS5在肝细胞性肝癌中的表达及其临床意义

Expression of long non-coding RNA GAS5 in hepatocellular carcinoma tissues and its clinical significance

目的探讨长链非编码RNA中的生长停滞敏感基因5(GAS5)mRNA在肝细胞性肝癌组织中的表达及其临床意义。方法回顾性收集2013年9月至2016年7月期间于重庆医科大学附属第一医院行根治性切除手术、术后病理学检查证实为原发性肝细胞性肝癌的120例患者的癌组织及其癌旁组织,应用实时荧光定量PCR(RT-PCR)法检测两类组织中GAS5 mRNA的表达,并分析其表达与肝细胞性肝癌患者临床病理学特征的关系,同时探索其对肝细胞性肝癌患者预后的影响。结果肝细胞性肝癌组织中GAS5 mRNA的表达水平低于癌旁组织(P<0.01)。GAS5 mRNA的表达与肿瘤直径、肿瘤数量、术前甲胎蛋白(AFP)水平、淋巴结转移、TNM临床分期和肿瘤分化程度均相关(P<0.05)。Cox比例风险回归模型结果表明,GAS5 mRNA的表达与预后有关(P<0.05)。结论 GAS5 mRNA有望成为肝细胞性肝癌早期诊断和预后判断的标志物。

Objective To investigate the expression of growth arrest-specific 5(GAS5) mRNA and its clinical significance in hepatocellular carcinoma. Methods The expression of GAS5 mRNA in the hepatocellular carcinoma tissues and corresponding adjacent tissues were detected by real time-PCR. The relationship between the expression of GAS5 mRNA and clinicopathological characteristics were analyzed by SPSS 19.0 software. Results The expression of GAS5 mRNA in hepatocellular carcinoma tissues was significantly lower than that of the adjacent tissues(P<0.01). The expression of GAS5 mRNA was related to tumor size, tumor number, lymph node metastasis, clinical TNM stage, alpha fetoprotein level, and tumor differentiation(P<0.05). Cox hazard model results showed that low expression of GAS5 mRNA was associated with poor prognosis(P<0.05). Conclusion GAS5 mRNA is expected to be a diagnostic and prognostic marker for patients with hepatocellular carcinoma.