高效液相色谱法测定番泻甙及其代谢产物番泻素

Determination of Sennosides and Degraded Products in the Process of Sennoside Metabolism by HPLC

采用高效液相色谱法分离测定番泻甙A和B及其代谢产物番泻素A和B,其色谱条件为:色谱柱SpherisorbC18不锈钢柱(250mm×4 6mmi d ,10μm),柱温40℃;检测波长360nm;流动相A为1 25%(体积分数)乙酸水溶液,流动相B为甲醇,线性梯度程序为100%A20min100%B。该方法快速、准确,能够对番泻甙的整个代谢过程进行实时分析。

A method for the separation and determination of sennosides A and B and the main composition (sennidins A and B) in degraded products of sennosides by linear gradient high performance liquid chromatography has been developed. Separation conditions were as follows: column, a Spherisorb C_18 column (250 mm×4.6 mm i.d., 10 μm); column temperature, 40 ℃; detection wavelength, 360 nm; mobile phase A, 1.25% acetic acid aqueous solution; mobile phase B, methanol; linear gradient, 100%A20 min100%B. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of real-time analysis in the process of sennoside metabolism.