小儿重症肺炎淋巴细胞凋亡的临床意义

Significance of lymphocytes apoptosis in severe pediatric pneumonia

目的 探讨小儿重症肺炎淋巴细胞凋亡的临床意义。方法 对 2 2例重症肺炎患儿分别在第 1天、第 5天采用流式细胞术检测其外周血中淋巴细胞早期凋亡率、晚期凋亡及坏死率 ,并与 2 1例普通肺炎患儿进行比较。结果 第 1次 ,重症肺炎患儿外周血淋巴细胞早期凋亡率为 (19 76±13 87) % ,晚期凋亡及坏死率为 (6 16± 6 6 0 ) % ,与普通肺炎组 [(11 14± 3 1) %和 (2 4 6± 2 72 ) % ]比较 ,差异均具显著性意义 (t=2 78,P <0 0 1;t=2 ,2 1,P <0 0 5 ) ;第 2次 ,重症肺炎患儿外周血淋巴细胞早期凋亡率为 (16 5 6± 13 6 ) % ,晚期凋亡及坏死率为 (2 79± 2 81) % ,与普通肺炎组 [(6 6± 1 9) %和 (1 4 3± 1 5 8) % ]比较 ,差异均具显著性意义 (t=3 32 ,P <0 0 1;t=2 0 3,P <0 0 5 )。结论 外周血淋巴细胞凋亡在重症肺炎患儿疾病的演变过程中可能起着一定的作用 ,提示对淋巴细胞凋亡的调控可能有助于疾病的恢复。

Objective To explore significance of lymphocytes apoptosis in severe pediatric pneumonia.Methods Early apoptosis rate,end stage apoptosis and death rate of peripherial blood lymphocytes in 1 day and 5 day were measured with flow cytometry in 22 cases of severe pediatric pneumonia and compared with 21 of common pneumonia.Results In first time,early apoptosis rate,end stage apoptosis and death rate of peripherial blood lymphocytes in severe pediatric pneumonia were (19.76±13.87)% and (6.16±6.6)% respectively. Both of them were higher than that of control[(11.14±3.1)%,(2.46±2.72)%],(t=2.78,P<0.01,t=2.21,P<0.05).In second time,early apoptosis rate was (16.56±13.6)%,end stage apoptosis and death rate was (2.79±2.81)%.Both of them were higher than that of controls[(6.6±1.9)%,(1.43±1.58)%],(t=3.32,P<0.01,t=2.03,P<0.05).Conclusion The peripherial blood lymphocytes apoptosis takes a certain effect during the prognosis of severe pediatric pneumonia,It may be helpful to regulate the lymphocytes apoptosis for the recovery of the disease.