姜黄素对免疫性前列腺炎大鼠肿瘤坏死因子-α、核因子-κB、环氧合酶-2表达的影响

Effects of curcumin on the expression of TNF-α,NF-κB,COX-2 in rats with autoimmune prostatitis

目的:探讨姜黄素对免疫性前列腺炎大鼠前列腺组织肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、核因子-κB(nuclear factorκB,NF-κB)、环氧合酶-2(cyclooxygenase-2,COX-2)表达的影响。方法:将60只SD雄性大鼠随机分为4组:正常对照组、模型组、姜黄素腹腔注射组及塞来昔布组(阳性对照组),每组15只。大鼠腹腔注射百白破疫苗同时皮下多点注射经过乳化的前列腺蛋白提纯液和免疫佐剂构建免疫性前列腺炎模型。造模成功后,连续给药四周。酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测前列腺组织中TNF-α的表达,免疫组织化学法测定前列腺组织NF-κB(p65)水平变化,免疫荧光测定前列腺组织COX-2水平变化,Western blot法测定前列腺组织NF-κB(p65)及COX-2的表达。结果:模型组大鼠前列腺组织中TNF-α(F=382.730,P=0.000)、NF-κB(p65)(F=56.230,P=0.000)、COX-2(F=122.290,P=0.000)的表达明显高于其他3组。姜黄素治疗组大鼠前列腺组织中TNF-α、COX-2表达明显低于塞来昔布治疗组(P<0.01),而NF-κB(p65)的表达无明显差异(P=0.059)。结论:姜黄素对免疫性前列腺炎大鼠有一定的治疗作用,其机制可能与降低前列腺组织中炎性细胞因子TNF-α、NF-κB(p65)、COX-2水平有关。

Objective:To investigate the influence of curcumin on the expression of tumor necrosis factor-α(TNF-α),nuclear factor κB(NF-κB)(p65)and cyclooxygenase-2(COX-2)in in rats with autoimmune prostatitis. Methods:Sixty male Sprague Dawley rats were equally and randomly divided into four groups:normal control group,model group,curcumin intraperitoneal injection group and celecoxib group(positive control group). The experimental autoimmune prostatitis rat model was made by intraperitoneal injection of vaccine and multiple hypodermic injection of prostate extract mixed with adjuvants. After the experimental animal model being estab-lished,each group was treated for four weeks. The levels of TNF-α in prostate tissues were detected by ELISA assay. The expressions of NF-κB(p65)and COX-2 in rat prostate tissues were respectively measured by immunohistochemistry,immunofluorescence and Western blot. Results:Levels of TNF-α(F=382.73,P=0.000),NF-κB(p65)(F=56.23,P=0.000)and COX-2(F=122.29,P=0.000)in prostate tissues were significantly higher in model group than in the other groups. Expressions of TNF-a and COX-2 were obviously lower in curcumin intraperitoneal injection group than in positive control group(P<0.01);no significant difference in the expression of NF-κB(p65) was observed between the two groups(P =0.059). Conclusion:Curcumin is effective in treating rats with autoimmune prostatitis,and the mechanism may be associated with the reduction of the cytokines TNF-α,NF-κB(p65)and COX-2 in prostate tissues.