改良型小鼠肾移植动物模型的建立

Establishment of the modified mouse kidney transplantation model

目的:建立一种操作简便、手术时间短、效果确切的小鼠肾移植模型。方法:静脉采用套扎式吻合,将供肾下腔静脉穿过导管,外翻套扎于导管,将其插入受体肾静脉内,套扎受体肾静脉于腔静脉外,完成静脉端端吻合;动脉采用供体腹主动脉与受体腹主动脉端侧吻合,供体膀胱瓣与受体膀胱吻合。结果:开放血管后供肾颜色正常,动脉搏动正常,静脉充盈好,成功建立小鼠肾移植模型,静脉吻合(10±5)min,动脉吻合(10±5)min,膀胱瓣吻合2~5 min,总手术时间40~60 min。结论:采用改良的套扎式原位静脉吻合和膀胱瓣吻合,可以降低手术难度,缩短手术时间,成功建立小鼠肾移植模型。

Objective:To establish a modified mouse kidney transplantation model with simple operation,short operation time and exact effect. Methods:The end to end anastomosis was perform on the veins,the donor kidney vena cava was evaginated after inserting catheter,and the evaginated vena cava was put into the receptor renal vein like sleeve. The abdominal aorta of the donor was anasto-mosed end-to-side to the abdominal aorta of the recipient. The donor bladder patch was anastomosed with the recipient bladder.Results:After open the blood vessels,the renal color was normal,the arterial pulsation was normal,the vein filling was good,and the kidney transplantation model of the mouse was successfully established. The venous anastomosis was(10±5)min,the arterial anastomosis was(10±5)min,and the bladder flap anastomosed was 2-5 min. Total operation time was 40-60 min. Conclusion:Using the modified type of venous anastomosis and the anastomosis of the bladder flap can reduce the difficulty and shorten the operation time. The mouse kidney transplantation model could be established successfully.