TLC-UV测定水飞蓟宾单磷酸酯钠的含量

Determination of sodium salt of 11-phosphate by TLC-UV spectrophotometry

目的 :为了准确测定水飞蓟宾单磷酸酯钠的含量。方法 :采用薄层层析对水飞蓟宾和水飞蓟宾单磷酸酯钠进行分离 ,用紫外分光光度法测定水飞蓟宾单磷酸酯钠的含量 ,测定波长 2 88nm。结果 :线性范围 5～30 μg/mL ,相关系数为 0 .9996 ,平均回收率为 98.93% ,RSD为 1.12 %。 结论 :本法能有效消除水飞蓟宾对测定的干扰 。

Objective: To determine the content of sodium salt of 11 phosphate. Methods: Silybin and sodium salt of 11 phosphate were separated by TLC. The content of sodium salt of 11 phosphate in solution was determined by UV spectrophotometry. The detection wavelength was 288nm. Results: The linear rang was 5～30μg/mL(r =0.999 6).The recovery was 98.93%(n =5),RSD was 1.12%. Conclusion: The method can eliminate the interference of silybin , the result was accurate.