真菌诱导子对长春花愈伤组织中吲哚生物碱积累的影响

Effects of fungal elicitors on accumulation of indole alkaloids in Catharanthus roseus calli

目的 研究分别来源于镰刀菌 Fusarium solani和黑曲霉 Aspergillum niger的真菌诱导子 (fungal elici-tors)对长春花愈伤组织中吲哚生物碱积累的影响。方法 用真菌诱导子对长春花愈伤组织进行诱导处理后提取吲哚总碱 ,并用 RP- HPL C法测定其中阿玛碱和长春质碱含量。结果 两种真菌诱导子对吲哚总碱及其中阿玛碱和长春质碱的积累均有较明显的正向调节作用 ,并且确立了真菌诱导子最佳处理时间。

Object To investigate the effects of fungal elicitors derived from the fungi Fusarium solani and Aspergillum niger on the accumulation of indole alkaloids in Catharanthus roseus calli. Methods The total indole alkaloid was extracted after the calli were treated with fungal elicitors. Then, the determination of ajmalicine and catharanthine was carried out by RP HPLC. Results The two fungal elicitors stimulate the accumulation not only the total indole alkaloid but the ajmalicine and catharanthine. The optimal exposure time of the two fungal elicitors for different kinds of indole alkaloid was investigated. Conclusion The two fungal elicitors have obvious effect on the accumulation of indole alkaloid in C. roseus calli.