葫芦巴丸加减方对子宫内膜异位症大鼠相关神经生长因子的影响

Effects of Fenugreek bolus pill on the expression of nerve growth factor in rat model of endometriosis

目的:建立子宫内膜异位症大鼠模型,探讨葫芦巴丸加减方药理作用及对于子宫内膜异位症模型大鼠神经生长因子的影响。方法:选用雌性SD大鼠54只,随机分为假手术组、空白模型组、阳性药物对照组(孕三烯酮组)和中药低、中、高剂量组。采用自体移植法建立子宫内膜异位症大鼠模型。葫芦巴丸加减方治疗后取大鼠异位子宫内膜,免疫组化法检测异位内膜中神经生长因子(NGF)、络氨酸激酶(trkA)、P75神经营养素受体(P75NTR)的表达。结果:葫芦巴丸加减方可以有效降低异位内膜NGF、trkA、P75 NTR的表达水平(P<0.01)。并且在中药治疗组低、中、高剂量组中比较,中剂量组对NGF和P75 NTR的表达水平结果优于低、高剂量组(P<0.05)。结论:葫芦巴丸加减方治疗子宫内膜异位症的可能机制,是通过抑制NGF、trkA、P75 NTR等神经生长因子起作用。

Objective:By establishing a rat model of endometriosis(EMT)to explore the pharmacologic mechanism of Fenugreek bolus pill and observe the its effects on the expression of NGF,trkA and P75 NTR in rats.Methods:A total of 54 female SD rats were selected and randomly divided into the sham operation group,blank model group,positive drug control group(gestrienone group),low dose,medium dose and high dose groups of traditional Chinese medicine.A rat model of EMT was established by autologous transplantation.After treatment,the expression of NGF,trkA and P75 NTR in the eutopic and ectopic endometrium of rats were detected by immunohistochemistry.Results:Fenugreek bolus pill can effectively reduce the expression levels of ectopic endometrial NGF,trkA and P75 NTR(P<0.01).In addition,compared with the low-dose and high-dose groups,the results of the medium-dose group were better than the above two groups(P<0.05).Conclusion:The mechanism of Fenugreek bolus pill in treating EMT may alleviate pain by inhibiting NGF,trkA,P75 NTR and other nerve growth factors.