摘要
目的 探讨RhoA在胃癌细胞系中的表达及其作用。方法 Westernblot方法检测RhoA在多株人消化道肿瘤细胞系中的表达 ;构建RhoA反义核酸真核表达载体 ,脂质体法转染至高表达RhoA的人胃癌细胞系AGS ,MTT比色法观察细胞生长 ,流式细胞仪检测细胞周期分布。结果 10株肿瘤细胞系中RhoA蛋白表达均显著高于永生化正常人肠黏膜细胞系HIEC。转染RhoA反义核酸真核载体后 ,AGS细胞中RhoA蛋白表达降低 ,细胞生长受到抑制 ,细胞周期分析显示聚集在S期的细胞明显增多。结论 RhoA蛋白在消化道肿瘤细胞系中表达明显增高 。
Objective To study the expression and possible function of RhoA in human gastric cancer cell lines. Methods The expression of RhoA in human gastroi ntestinal cancer cell lines was detected by Western blot. Antisense plasmid of R hoA was constructed by pGEFL and transferred into gastric cancer cell line AGS by lipofectamine. Cell survival was examined by MTT assays, and cell cycle was d etected by flow cytometry. Results The expression of RhoA protein in 10 differen t kinds of human cancer cell lines was much higher than that in immortalized hum an intestinal epithelial cell line. After being transfected with antisense RhoA, with the decrease in RhoA protein expression, the growth rate of AGS was inhibi ted ,and the number of cells in S phase was increased by 14%. Conclusion RhoA is overexpressed in many human cancer cell lines. Some of the malignant cha racteristics of a gastric cancer cell line can be partially reversed by inhibiti ng RhoA expression.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2004年第1期26-29,共4页
Chinese Journal of Oncology
基金
国家自然科学基金主任基金资助项目(30 1 40 0 2 6)