抗骨松穴位贴剂对实验性骨质疏松大鼠的防治作用

Preventive and Treating Effects of Anti-Osteoporosis Acupoint Plaster on the Rats with Experimental Osteoporosis

目的 探讨抗骨松穴位贴剂 (AOAP)拮抗实验性骨质疏松 (OP)、“破骨大于成骨”、逆转“阳杀阴藏”的机理。方法 采用注射地塞米松 (Dex)建立OP模型 ,用AOAP外贴穴位、非经非穴位 ,并与口服补肾方药比较治疗OP ,以血清T/E2 、骨密度、CT/PTH ,骨形态计量学参数TBV、TBS、TFS、LB GR、AFS、MAR、BFR、TRS的变化为衡量指标。结果 AOAP能提高T/E2 、CT/PTH和骨密度 ,升高TBV、TBS、TFS、LBGR、AFS、MAR、BFR ,降低TRS。结论 AOAP可以通过“多位点相关集合效应”调控影响骨代谢的内分泌 ,提高T/E2 、CT/PTH水平 ,可以逆转OP“钙搬家” ,拮抗骨细胞“阳杀阴藏”的过程 。

Objective To investigate the effects of Anti Osteoporosis Acupoint Plaster (AOAP) on experimental osteoporosis (EO), and its mechanism of antagonizing the development of osteoporosis. Methods The rat model was established by intramuscular dexamethasone injection. The model rats were treated with AOAP by plastering the plaster on the selected acupoints and on the selected non acupoints, with drugs for tonifying the kidney as a control. The serum T/E2, bone density, CT/PTH, and the bone morphometrical indexes, such as TBV, TBS, TFS, LBGR, AFS, MAR, BFR, TRS were used as the criteria in observing the effects of AOAP.Results AOAP could increase the bone density and the levels of serum T/E2 and CT/PTH, raise the levels of TBV, TBS, TFS, LBGR, AFS, MAR and BFR, and reduce the level of TRS.Conclusion AOAP can regulate and control the incretion related with bone metabolism by multiposition correlational aggregative effect, raise the levels of T/E2 and CT/PTH, avoid the calcium loss in osteoporosis and increase bone density.