小柴胡汤对实验性肝损伤小鼠Th1/Th2免疫平衡的影响

Effect of Xiaochaihu Decoction(小柴胡汤)on the Th1/Th2 Immune Balance of Experimental Hepatic Injury in Mice

目的 :研究小柴胡汤对实验性肝损伤小鼠 Th1/ Th2免疫平衡的影响。方法 :以卡介苗 (BCG)加脂多糖 (L PS)诱导小鼠免疫性肝损伤 ,模型小鼠给小柴胡汤水煎剂灌胃 ,以联苯双酯片 (BDD)作阳性对照药 ,并设空白对照。 10天后处死小鼠 ,用酶联免疫吸附法 (EL ISA)测定 Th1型细胞因子干扰素γ(IFN-γ)和 Th2型细胞因子白介素 - 4 (IL - 4 )的血清浓度。结果 :模型组 Th1型细胞因子 IFN-γ浓度显著低于正常对照组 (P <0 .0 1) ,Th2型细胞因子 IL - 4的血清浓度显著高于正常对照组(P <0 .0 1) ,Th1/ Th2平衡向 Th2方向飘移 ;小柴胡汤治疗组 IFN-γ浓度显著高于肝损伤模型组 (P <0 .0 1) ,IL - 4浓度显著低于肝损伤模型组 (P <0 .0 5 ) ,Th1/ Th2平衡飘移得到纠正。结论 :小柴胡汤可使实验性肝损伤小鼠血清 IFN-γ含量升高 ,IL- 4含量下降 ,从而使 Th1/

Objective:To study the effect of Xiaochaihu Decoction(XD) on the immune function of mice with experimental hepatic injury.Methods:Bacille Calmette Guerin(BCG) and Lipopolysaccharide(LPS) were used to set up the mice model of hepatic injury.And then the mice were treated with XD.Mice treated with Biphenyl dimethyl dicarboxylate(BDD) and the normal mice were set up as the control groups.10 days later,the serum concentration of Th1 type cytokine IFN γ and Th2 type cytokine IL 4 were detected by Enzyme linked immunosorbent assay(ELISA).Results:In the model group,serum concentration of IFN γ was much lower than that in the normal control group(P<0 01),and serum concentration of IL 4 was much higher than that in the control group(P<0 01).And the Th1/Th2 immune balance was broken and shifted towards Th2.In the treatment group,serum concentration of INF γ was much higher than that in the model group(P<0 01),and serum concentration of IL 4 was much lower than that in the model group(P<0 05),the Th1/Th2 immune balance was regained.Conclusion:XD could increase the serum concentration of IFN γ and decrease the serum concentration of IL 4 in mice model of experimental hepatic injury,thus to correct the imbalance of Th1/Th2 immune function.