摇瓶发酵烃降解率测定方法的研究

Preliminary Study of Determining Method of the Hydrocarbon Degrading Rate in Shaking Flask

对两种常规测定微生物发酵石油烃摇瓶中残余石油烃的质量的常用方法,即重量法和比色法进行了比较研究。实验结果表明:摇瓶发酵后过滤-洗涤的方法明显比离心-萃取的方法要便捷;比色法测得的烃降解率偏小,但在初筛时比其他方法便捷、精确度高。因此,初筛烃降解菌测定摇瓶发酵烃降解率时一般采用过滤-洗涤-比色法。

Under current experimental conditions,two methods- colorimetric method and gravimetric method were used to measure the rate of residual hydrocarbons in shake flask and were then analyzed comparatively.And the results show that the method of filter-washing was more convenient than that of centrifugal extraction in the treatment of samples.The measured degrading rate by colorimetric method was lower than that by the gravimetric method.Nevertheless in the process of the primary screening,the colorimetric mathod proved to be more convenient than the gravimetric method,which will be employed unless accurate screening or breeding of degrading-alkane bacteria are carried out.