摘要
目的:建立陈皮饮片标准汤剂的指纹图谱并对其进行质量评价。方法:制备15批陈皮饮片标准汤剂,采用HPLC测定陈皮饮片标准汤剂中橙皮苷的含量;采用紫外光谱法(UV)和红外光谱法(IR)建立陈皮饮片标准汤剂的指纹图谱;利用相关系数法和双指标序列分析法对不同批次陈皮饮片标准汤剂的光谱图进行比对。结果:15批陈皮饮片标准汤剂中橙皮苷的质量分数0. 82%~2. 60%,出膏率实测值32. 02%~46. 11%;与紫外和红外对照指纹图谱相比,各批次陈皮饮片标准汤剂指纹图谱的相似度分别为> 0. 897和> 0. 942,双指标分析法结果显示各批次陈皮饮片标准汤剂的共有峰率均>62. 50%,变异峰率均<46. 67%。结论:该研究建立的质量评价方法可用于陈皮饮片标准汤剂的系统性评价,可为陈皮配方颗粒等相关制剂的质量标准制定提供参考。
Objective: To establish the fingerprint of standard decoction of Citri Reticulatae Pericarpium and evaluate its quality. Method: According to the preparation conditions of the standard decoction,15 batches of standard decoction of Citri Reticulatae Pericarpium were prepared. HPLC was employed to determine the content of hesperidin in this standard decoction. Ultraviolet spectroscopy( UV) and infrared spectroscopy( IR) were used to establish the fingerprint of standard decoction of Citri Reticulatae Pericarpium. The correlation coefficient method and double index sequence analysis method were used to compare and analyze the spectra of different batches of this standard decoction. Result: The content of hesperidin in 15 batches of this standard decoction were 0. 82%-2. 60%,and the measured value of dry extract rate was 32. 02%-46. 11%. Compared with ultraviolet and infrared control fingerprint,the fingerprint similarities of the standard decoction of each batch were > 0. 897 and > 0. 942,respectively. The double index analysis results showed that the common peak ratio was more than 62. 50%,variation peak ratio was less than 46. 67%. Conclusion: The quality evaluation method established in this study can be used for systematic evaluation of standard decoction of Citri Reticulatae Pericarpium,and it can provide theoretical reference for the formulation of quality standard of Citri Reticulatae Pericarpium dispensing granules and other related preparations.
作者
刘涛
张宇雪
张佳
谢梦蝶
潘春晖
杨慧
邓燕君
蒋天宇
徐玉玲
LIU Tao;ZHANG Yu-xue;ZHANG Jia;XIE Meng-die;PAN Chun-hui;YANG Hui;DENG Yan-jun;JIANG Tian-yu;XU Yu-ling(College of Pharmacy and Biological Engineering,Chengdu University,Chengdu 610106,China;Sichuan Decheng Animal Health Products Co.Ltd.,Deyang 618100,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第6期136-143,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
四川省科技厅2018年科技项目(2018ZR0107
2018TJPT0049)
关键词
陈皮
标准汤剂
红外指纹图谱
紫外指纹图谱
双指标序列分析法
橙皮苷
出膏率
Citri Reticulatae Pericarpium
standard decoction
infrared fingerprint
ultraviolet fingerprint
double index sequence analysis method
hesperidin
dry extract rate