两面针果壳的化学成分分析及活性

Analysis of Chemical Constituents and Activity on Zanthoxylum nitidum Husk

目的:利用高效液相色谱-线性离子阱/静电场轨道阱高分辨质谱(HPLC-LTQ/Orbitrap-MS)技术对两面针非药用部位果壳中的生物碱类成分进行分析和鉴定,并对其抗氧化活性进行研究,为两面针植物更深入地研究开发提供科学依据。方法:采用HPLC-LTQ/Orbitrap-MS采集数据后,对各色谱峰的质谱图高分辨一级和二级质谱数据进行解析,与文献数据库进行对比,对各色谱峰进行结构推测和确认。采用1,1-二苯基-2-三硝基苯肼(DPPH)清除自由基能力法及2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除能力对两面针果壳样品抗氧化活性进行研究。结果:从两面针果壳中共鉴定了25个生物碱类化合物,主要化学成分为异喹啉类生物碱(两面针碱、鹅楸掌碱、木兰箭毒碱),吡咯类生物碱(别隐品碱、氧化苦参碱、氧化槐果碱),喹啉类生物碱(木兰花碱、氯化两面针碱),有机胺类生物碱(γ-山椒素)。DPPH清除自由基能力法及ABTS自由基清除能力对其抗氧化活性进行了研究,结果显示在测定的浓度范围内,抗氧化活性均随着试样浓度的增加而逐渐增强,呈现良好的剂量依赖性。结论:该文对两面针果壳进行了化学成分及活性的研究,研究证明其具有多种生物碱类成分,并具有较好的抗氧化活性,为两面针植物更深入地研究开发提供了参考。

Objective:To analyze and identify the non-medicinal parts in Zanthoxylum nitidum husk by HPLC-LTQ/Orbitrap-MS,and study the antioxidant activity,in order to provide the scientific basis for further research and development of Z.nitidum.Method:Data is collected by HPLC-LTQ/Orbitrap-MS,and highresolution MS and MS2 spectra of mass spectrogram of chromatographic peaks were analyzed and compared with the literature database.The structure of each chromatographic peak was calculated and confirmed.The anti-oxidative activity of the Z.nitidum husk was studied by DPPH scavenging free radical ability and ABTS free radical scavenging ability.Result:Twenty-five alkaloids were identified from Z.nitidum husk.The main constituents were isoquinoline alkaloids(nitidine,liriodenine,magnocurarine),pyrrolidine alkaloid(allocryptopine,oxymatrine,oxysophocarpine),quinoline alkaloid(magnoflorine,nitidine chloride),and organic amine alkaloids(γ-sanshool).Antioxygenic activity was studied by DPPH scavenging free radical ability and ABTS free radical scavenging ability.The results showed that they were within the measured concentration range,the antioxidant activity increased with the rise of the sample concentration,a good dose dependence was presented.Conclusion:In this paper,the chemical constituents and the activity Z.nitidum husk were studied.Studies have shown a variety of alkaloids,with a good antioxidant activity.This study provides a reference for further research and development of Z.nitidum.