基于“入腹知性”理论的黄芩干预大鼠肠道菌群多样性的探讨

Intestinal Microflora Diversity of Rats Treated with Scutellariae Radix Based on Theory of "Insight into Abdomen"

目的:探讨黄芩对生理状态下大鼠肠道菌群多样性的变化,分析并判定黄芩药性的归属。方法:利用16S rRNA高通量基因测序技术对黄芩给药(10 g·kg-1)干预后的大鼠盲肠内溶物进行检测,将肠道菌群分类单元(OTUs)数量,丰富度与多样性指数及差异菌门与菌属进行综合性分析,并利用网络可视化手段找寻差异菌门与菌属之间的关联性。结果:基于Illumina Miseq平台,与空白组相比,黄芩给药组大鼠肠道菌群的OTUs数量减少,丰富度与多样性指数降低;拟杆菌门(Bacteroidetes)和螺旋体菌门(Spirochaetes)显著性上调(P <0. 01),厚壁菌门(Firmicutes),放线菌门(Actinobacteria),变形菌门(Proteobacteria)及软壁菌门(Tenericutes)显著性下调(P <0. 01),24个差异菌属中有17个显著性下调(P <0. 05,P <0. 01),7种显著性上调(P <0. 01)。联合网络可视化分析确定影响程度最大的菌门为拟杆菌门与厚壁菌门,其中厚壁菌门与其余菌门多呈现出负相关的关系,与螺旋体菌门存在最强负相关性;拟杆菌门与变形菌门、厚壁菌门呈正相关的关系,与放线菌门呈现负相关的关系,与其余菌门无相关性相关性;差异菌属多富集于瘤胃球菌属(Ruminococcus),帕拉普氏菌属(Paraprevotella),普雷沃菌属(Prevotella)及颤螺旋菌属(Oscillospira)。结论:黄芩可降低肠道菌群多样性,抑制机体的菌群代谢功能,因此从菌群多样性角度推断黄芩的药性为寒凉。

Objective:To explore the effect of Scutellariae Radix on the diversity of intestinal flora in rats under physiological conditions,in order to determine the property of Scutellariae Radix property.Method:16 S rRNA high-throughput gene sequencing technique was used to detect the cecum solutes of rats treated with Scutellariae Radix(10 g·kg-1).The number,richness and diversity index of the intestinal flora taxon(OTUs)and the differential phylum and genus were comprehensively analyzed.The network visualization was used to find the correlation between differential phylum and genus.Result:Based on the Illumina Miseq platform,compared with the blank group,the number of OTUs and the index of richness and diversity of the intestinal flora of the rats treated with Scutellariae Radix decreased.Bacteroidetes and Spirochaetes were significantly up-regulated(P<0.01),while Firmicutes,Actinobacteria,Proteobacteria and Tenericutes were significantly down-regulated(P<0.01).Among the 24 differential bacteria genera,17 were significantly down-regulated(P<0.05,P<0.01),while 7 species were significantly up-regulated(P<0.01).On the basis of the network visualization analysis,the most influential phylua were Bacteroides and Firmicutes,in which Firmicutes showed a negative correlation with the rest of phylum,with the strongest negative correlation with spirochetes.There was a positive correlation between Bacteroides,Proteobacteria and Firmicutes,but a negative correlation with Actinobacteria,and no correlation with other phylum.Most of the differential bacteria were enriched in Ruminococcus,Paraprevotella,Prevotella and Oscillospira.Conclusion:Scutellariae Radix can reduce the diversity of intestinal flora and inhibit the metabolism of the body,so its property is cold.