南苍术与北苍术的燥性效应比较

Comparison of Dryness Effect Between Atractylodes lancea and A.chinensis

目的:探讨南苍术和北苍术的燥性效应强弱。方法:将60只正常健康SD大鼠随机分组为6组(每组10只),分别为生理盐水组,大豆油组,南苍术低剂量组(剂量46. 25 mg·kg-1·d-1),南苍术高剂量组(剂量500 mg·kg-1·d-1),北苍术低剂量组(剂量46. 25 mg·kg-1·d-1),北苍术高剂量组(剂量500 mg·kg-1·d-1),给药体积均为0. 01 m L·g-1,灌胃给药21 d。以大鼠日均饮水量、颌下腺形态组织、尿量和肾脏中水通道蛋白2(AQP2)表达量、全血黏度为苍术燥性效应的药效评价指标,观察给予等剂量南苍术挥发油和北苍术挥发油对大鼠燥性效应的影响。结果:与大豆油组相比较,长期服用高剂量南苍术挥发油和北苍术挥发油均能使大鼠日均饮水量、尿量、全血黏度增加,AQP2表达量降低,颌下腺腺泡萎缩;但二者并无统计学差异;而低剂量南苍术挥发油和北苍术挥发油对大鼠燥性效应影响不显著。结论:等剂量的南苍术挥发油和北苍术挥发油表现出来的燥性药效无显著性差异,初步证明了临床上南苍术和北苍术通用的合理性,同时为苍术的临床合理用药提供了实验依据。

Objective:To investigate the dryness effect of Atractylodes lancea and A.chinensis.Method:Sixty normal and healthy SD rats were randomly divided into 6 groups(10 in each group),including normal saline group,soybean oil group,low-dose(46.25 mg·kg-1·d-1)group and high-dose(500 mg·kg-1·d-1)group of A.lancea,low-dose(46.25 mg·kg-1·d-1)group and high-dose(500 mg·kg-1·d-1)group of A.chinensis,the dosing volume was 0.01 m L·g-1,and the drug was administered orally for 21 days.Taking average daily water intake,submandibular gland tissue,urine volume and expression of aquaporin 2(AQP2)in the kidney,and whole blood viscosity as the evaluation indexes,the dryness effect of longterm administration of equal doses of volatile oil from A.lancea and volatile oil from A.chinensis on rats was observed.Result:Compared with the soybean oil group,long-term administration of high doses of volatile oil from A.lancea and volatile oil from A.chinensis could significantly increase average daily water intake,urine volume and whole blood viscosity;decrease the expression of AQP2,and atrophy the acini of submandibular gland,but there was no significant difference between the two groups.Effects of volatile oil from A.lancea and A.chinensis with low dose on dryness of rats were not significant.Conclusion:There is no significant difference between the dryness effect of volatile oil from A.lancea and A.chinensis in the same dose.It is proved that the rationality of A.lancea and A.chinensis are universal in clinical practice,and this study provides experimental basis for rational use of Atractylodis Rhizoma.