霍山石斛的抗炎作用

Anti-inflammatory Effect of Dendrobii Huoshanense Herba

目的:观察霍山石斛的抗炎作用。方法:60只昆明种小鼠,随机分为正常组、模型组、石斛高、中、低剂量组(4,2,1 g·kg-1),醋酸地塞米松组(醋酸地塞米松,0. 01 g·kg-1);连续灌胃给药14 d;末次给药1 h后,于小鼠右耳的耳中部两侧滴二甲苯20μL建立耳肿胀模型。1 h后断头处死所有小鼠,计算各组小鼠耳肿胀率。健康SD大鼠36只,分别为正常组、模型组、霍山石斛高、中、低(2. 8,1. 4,0. 7 g·kg-1)剂量组、醋酸地塞米松组。连续灌胃给药7 d。末次灌胃给药1 h后,于各组大鼠的右后肢足趾皮下注射10%新鲜鸡蛋清建立足肿胀模型。分别于0,0. 5,1,2,3,4,5 h时,测量各大鼠的右后足周长,计算大鼠的足肿胀度。取SD大鼠60只,向双侧腹股沟皮下植入无菌干燥棉球,分组同上。第2天灌胃给药,连续14 d。末次给药后,取出棉球,计算肉芽肿抑制率;检测各组大鼠血清中肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL-1β),白细胞介素-2(IL-2),白细胞介素-6(IL-6),γ-干扰素(IFN-γ)含量。结果:与正常组比较,模型组耳肿胀率、足肿胀度显著升高(P <0. 01);与模型组比较,霍山石斛高、中、低剂量组均可明显降低小鼠耳肿胀率(P <0. 01);霍山石斛高、中剂量组足肿胀度明显降低(P<0. 05,P <0. 01);给药各组棉球肉芽干重显著下降(P <0. 01),胸腺、脾脏指数没有显著性变化;与正常组比较,模型组大鼠血清中炎症因子含量显著升高(P <0. 01);与模型组比较,霍山石斛高、中、低剂量组和醋酸地塞米松组炎症因子含量显著降低(P <0. 05,P <0. 01)。结论:霍山石斛能够有效抑制急、慢性炎症反应。

Objective:To observe the anti-inflammatory effect of Dendrobii Huoshanense Herba.Method:Totally 60 Kunming mice were randomly divided into normal group,model group,high,middle and lowdose Dendrobii Huoshanense Herba groups(DHS-H,DHS-M,DHS-L,4,2,1 g·kg-1)and dexamethasone acetate group(DXMS,0.01 g·kg-1).The rats were intragastrically administered for 14 days.After the last administration for 1 h,a total of 20μL of xylene was added to both sides of the right ear center of the mice to establish the ear swelling model.All of the mice were decapitated 1 h later,and the ear swelling inhibition rate of each group were calculated.Totally 36 healthy SD rats were divided into normal model,model group,DHS-H,DHS-M,DHS-L groups(2.8,1.4,0.7 g·kg-1)and DXMS acetate group.The rats were intragastrically administered for 7 days.One hour after the last intragastric administration,a foot swelling model was established through subcutaneous injection of 10%fresh egg white in the right hind limb toe of each group.The right hind paw circumference of each rat was measured at 0,0.5,1,2,3,4,5 h,and the paw swelling of the rats was calculated.Totally 60 SD rats were implanted subcutaneously with sterile dry cotton balls in the bilateral groin and grouped as above.All of the rats were intragastrically administered for 14 days since the next day.After the last administration,cotton balls were taken out,and the inhibition rate of granuloma was calculated.And the contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-2(IL-2),interleukin-6(IL-6),interferon-γ(IFN-γ)in the serum of each group were detected.Result:Compared with the normal group,the ear swelling rate,the foot swelling degree were significantly increased in the model group(P<0.01).Compared with the model group,the swelling rate of mouse ears in high,middle and low-dose Dendrobii Huoshanense Herba groups reduced significantly(P<0.01).The swelling degrees of the high and middle-dose groups were significantly decreased(P<0.05,P<0.01).The dry weight of cotton buds in each group was significantly decreased(P<0.01),with no significant change in thymus and spleen index.Compared with the normal group,the serum levels of TNF-α,IL-1β,IL-2,IL-6,and IFN-γin the model group were higher than those in the normal group(P<0.01).And compared with model group,the levels of TNF-α,IL-1β,IL-2,IL-6,and IFN-γin Dendrobii Huoshanense Herba group and positive drug group were decreased significantly(P<0.05,P<0.01).Conclusion:Dendrobii Huoshanense Herba could effectively inhibit acute and chronic inflammatory reactions.