不同色泽红参与水分和5-羟甲基糠醛含量的相关性分析

Correlation Between Color and Contents of Water and 5-Hydroxymethylfurfural in Ginseng Radix et Rhizoma Rubra

目的:探讨不同红参样品的色泽与水分和5-羟甲基糠醛(5-HMF)含量的相关性。方法:以白纸为背景日光下观察色泽;采用色差仪测定色度值,并计算色差值;采用烘干法测定水分;建立高效液相色谱法(HPLC)测定5-HMF的含量;采用Spearman和Pearson系数进行相关性分析。结果:红参的色泽呈黄白、土黄、棕黄色。色差仪测定和计算的L*,a*,b*,ΔE*有一定的范围,可以体现红参色泽。水分在4. 02%~8. 31%,符合2015年版《中国药典》的规定。5-HMF的含量从低于检测限(1. 985×10-4μg)到78. 97μg·g-1。相关性分析结果认为,L*,a*,b*和ΔE*值均与水分含量存在极显著的相关性,即色泽越深水分含量越高;而与5-HMF的含量无显著相关性。结论:作为炮制品的红参,其色泽的差异与其中的水分含量有关,而与中药材中普遍存在的色泽与5-HMF含量相关的现象不一致,说明药材经炮制后色泽的深浅与药材本身色泽变化的原因具有本质区别。因此,控制水分含量,对于保证红参色泽的稳定具有重要意义。该文应用色差仪对红参进行色泽的测定,系统地测定了其中5-HMF的含量,并提出水分含量对红参色泽的影响。

Objective:To explore the correlation between colors and contents of water and 5-hydroxymethylfurfural(5-HMF)in Ginseng Radix et Rhizoma Rubra(GRRR).Method:The colors were observed under sunlight on a sheet of white paper.The chroma value and color difference were determined and calculated by a colorimeter.Water content was determined by the oven-drying method.An HPLC method was established and used to determine the content of 5-HMF.The correlation was analyzed by the Spearman and Pearson analysis.Result:The colors of GRRR were yellowish-white,ocher-yellow,and yellowish-brown.The values of L*,a*,b*,andΔE*had a certain range,and could reflect the colors of GRRR.The content of water ranged from 4.01%to 8.31%and was in accordance with the requirement the Chinese Pharmacopoeia.The content of 5-HMF ranged from below the limit of detection(1.985×10-4μg)to 78.97μg·g-1.The results of correlation analysis shows significant correlations between L*,a*,b*,ΔE*values and water content,that is to say,the deeper the color was,the higher the content of water was,but with no significant correlation with 5-HMF content.Conclusion:The color of processed GRRR was related to water content but not related to 5-HMF content,which was not consistent with the correlation between color and 5-HMF content that generally existed in a series of raw Chinese medicinal materials.It indicated an essential difference between the color shade of processed Chinese medicinal materials and the cause of color change of raw Chinese medicinal materials.Therefore,it was significant to control water content for ensuring the stability of GRRR.This study determined the colors of GRRR by a colorimeter,systematically determined 5-HMF content,and proposed the effect of water contents on the color of GRRR.