藏族药川西獐牙菜化学成分与生态因子的相关性分析

Correlation Between Chemical Constituents and Ecological Factors of Tibetan Medicine Swertia mussotii

目的:建立川西獐牙菜的HPLC指纹图谱,研究不同产地样品化学成分与生态因子的相关性。方法:采用高效液相色谱法(HPLC),采用Wondasil C18色谱柱(4. 6 mm×250 mm,5μm),流动相甲醇-0. 2%磷酸溶液,梯度洗脱,柱温30℃,流速1. 0 m L·min-1,检测波长254 nm,建立川西獐牙菜的指纹图谱,结合国家药典委员会颁布的'中药色谱指纹图谱相似度评价系统2004A版'进行评价分析;并收集各产地样品的气候、土壤等生态因子数据,利用DPS数据处理系统,SPSS 21. 0统计软件对不同产地药材的化学成分与生态因子进行灰色关联度和双变量分析。结果:建立了川西獐牙菜药材的HPLC指纹图谱,共标定12个共有特征指纹峰,确定了7个峰的化学成分;川西獐牙菜中的獐牙菜苦苷、芒果苷等化学成分与生态因子具有显著的相关性,对化学成分影响较大的为气候因子中的月均温范围、年降水量和降水季节性,以及土壤因子中的土壤中有机碳比例和土壤酸碱度。结论:川西獐牙菜药材的化学成分与外部生态因子有一定的相关性,研究结果为该药材的人工种植及藏族药'生地性'理论诠释提供了参考依据。

Objective:The HPLC fingerprinting of Swertia mussotii was established to study the correlation between chemical components and ecological factors in different areas.Method:The fingerprint of S.mussotii was established by high performance liquid chromatography(HPLC),and the evaluation and analysis were made based on the'Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System 2004 A Edition'promulgated by the National Pharmacopoeia Commission.The analysis was carried out on a Wondasil C18 column(4.6 mm×250 mm,5μm),with methanol-0.2%phosphoric acid as the mobile phase for gradient elution,and the column temperature was set at 30℃.The flow rate was 1.0 m L·min-1,and the detection wavelength was set at 245 nm.And data on ecological factors in each sample habitat,such as climate and soil,were collected.The gray correlation and bivariate analysis were carried out on the chemical constituents and ecological factors of medicinal materials in different areas using DPS data processing system and SPSS 21.0 statistical software.Result:The HPLC fingerprint of S.mussotii was established,a total of 12 common fingerprint peaks were marked,and the chemical constituent of the seven peaks were determined.The chemical constituents,such as swertiamain and mangiferin of S.mussotii,were significantly correlated with ecological factors.Moreover,the chemical constituents were obviously affected by the monthly average temperature range,annual precipitation,precipitation seasonality in the climatic factors,the soil organic carbon ratio and soil p H in the soil factors.Conclusion:The chemical constituents of S.mussotii have a correlation with the external ecological factors,the findings could provide a basis for the artificial planting of the medicinal material and the scientific connotation of the'environment-based'theory for Tibetan medicines.