基于生物电阻抗评价大鼠脾虚水湿内停模型

Evaluation of Rat Model with Spleen Deficiency and Dampness Stagnancy Based on Bioelectrical Impedance

目的:通过生物电阻抗法(BIA)与传统指标评价复合因素诱导脾虚水湿内停模型的成模标准。方法:将40只大鼠分为空白组和模型组,每组20只。空白组大鼠给予正常饲料喂养;模型组大鼠采用饮食不节结合劳倦过度法制备大鼠脾虚水湿内停模型14 d。观察大鼠的一般状况,烘干法检测大鼠粪便含水率,称量法检测水负荷指数,酶联免疫吸附测定(ELISA)检测尿D-木糖排出量,血清总蛋白(TP),白蛋白(Alb)含量,蛋白免疫印迹法(Western blot)检测肾髓水通道蛋白1(AQP1)含量,同时运用实验动物体组成测定分析仪检测总含水率(TBW),细胞外含水率(ECF),细胞内含水率(ICF),脂肪量(FM),除脂肪量(FFM)和身体质量指数(BMI)等生物电阻抗指标。结果:与空白组比较,模型组大鼠逐渐消瘦,偶尔稀便,肛温基本不变,体质量,D-木糖排泄量,水负荷指数,TP和Alb含量显著降低(P <0. 01),粪便含水率和肾髓AQP1蛋白表达显著升高(P <0. 01);TBW,ECF和FFM升高,ICF,FM和BMI显著降低(P <0. 01)。结论:饮食不节结合劳倦过度的复合因素诱导脾虚水湿内停模型大鼠会出现体脂率降低和胃肠水液代谢功能的减退,生物电阻抗法可以更直观全面的反映成模情况。

Objective:To evaluate the model with spleen deficiency and dampness stagnancy by bioelectrical impedance analysis(BIA)and traditional indicators.Method:The forty rats were divided into blank group and model group,with 20 rats in each group.The rats in the blank group were fed with normal feed,the rats in model group were prepared with the spleen deficiency and dampness stagnancy model for 14 days.Observe the general condition of the rats,measure the water content of the feces in the dry method,measure the water load index by weighing method,and detect the urinary D-xylose excretion total protein(TP),albumin(Alb)content,by enzyme-linked immunosorbent assay(ELISA).Western blot analysis of renal aquaporin 1(AQP1)content,and the use of experimental animal body composition analyzer to determine the total water content(TBW),extracellular fluid(ECF),intracellular fluid(ICF),fat mass(FM),free fat mass(FFM)and body mass bioelectrical impedance index such as body mass index(BMI).Result:Compared with blank group,the rats in model group lost weight,gradually loose stools occasionally,the anus temperature was basically unchanged,body mass,D-xylose excretion,water load index,TP and Alb content decreased(P<0.01).The feces contained water,the rate and the content of AQP1 in the renal pulp were increased(P<0.01).The TBW,ECF and FFM were increased,and the ICF,FM and BMI were decreased(P<0.01).Conclusion:Rats with spleen deficiency and dampness stagnancy induced a combination of factors such as diet and excessive fatigue.The bioelectrical impedance method can be more intuitive and comprehensive.