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硫磺熏蒸对西洋参化学成分含量的影响及其鉴别方法探讨 被引量:5

Effect of Sulfur Fumigation on Content of Chemical Constituents in Panacis Quinquefolii Radix and Preliminary Study on Its Identification Method
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摘要 目的:阐明硫磺熏蒸对西洋参化学成分含量及品质的影响,探讨硫熏西洋参的鉴别方法。方法:采用HPLC-DAD测定西洋参中人参皂苷Rb1,Re,Rg1在硫熏前后的含量变化;采用微波消解法和电感耦合等离子体发射光谱串联质谱(ICP-MS)测定西洋参中11种无机元素在硫熏前后的含量变化;结合主成分分析方法对西洋参硫熏前后的品质差别进行定性区分。结果:3种人参皂苷类成分分离良好,在各自的质量浓度范围内与峰面积呈良好的线性,R2均≥0.999。硫磺熏蒸前,西洋参中人参皂苷Rg1,Re,Rb1的质量分数分别为(0.897±0.045),(13.092±0.639),(12.896±0.430)g·kg-1;硫磺熏蒸后,三者的质量分数分别为(0.807±0.032),(11.968±0.627),(11.752±0.696)g·kg-1;硫熏后,无机元素Cr,Mn,Co,Cu,As,Se,Ag,Cd,Sb,Pb和Fe的含量均有不同程度的增加;综合西洋参中3种有效成分和11种无机元素的含量进行主成分分析,根据三维散点分布结果能够对西洋参硫熏品和非硫熏品进行有效区分。结论:硫磺熏蒸后,西洋参中人参皂苷Re和Rb1的含量显著降低,但11种无机元素的含量均有所增加,提示硫磺熏蒸可能会对西洋参品质产生较大影响。 Objective:To elucidate the effect of sulfur fumigation on the content of chemical composition and quality of Panacis Quinquefolii Radix,and to discuss the identification method of sulfur fumigated Panacis Quinquefolii Radix.Method:The content changes of ginsenoside Rb1,Re,Rg1in Panacis Quinquefolii Radix were determined by HPLC-DAD before and after sulfur fumigation.The content changes of 11 inorganic elements in Panacis Quinquefolii Radix before and after sulfur fumigation were detected by microwave digestion method and inductively coupled plasma emission spectrometry tandem mass spectrometry(ICP-MS).The quality difference o Panacis Quinquefolii Radix before and after sulfur fumigation was qualitatively distinguished combined with the principal component analysis.Result:Three ginsenosides were well separated and showed good linearity with R2≥0.999.Before sulfur fumigation,the contents of ginsenoside Rg1,Re and Rb1in Panacis Quinquefolii Radix were(0.897±0.045),(13.092±0.639),(12.896±0.430)g·kg-1,respectively.After sulfur fumigation,the contents of these three components in Panacis Quinquefolii Radix were(0.807±0.032),(11.968±0.627),(11.752±0.696)g·kg-1,respectively.After fumigation,the contents of inorganic elements(Cr,Mn,Co,Cu,As,Se,Ag,Cd,Sb,Pb and Fe)from Panacis Quinquefolii Radix increased in varying degrees.According to the distribution results of three-dimensional(3D)scattered points,sulfur and non-sulfur fumigation products of Panacis Quinquefolii Radix could be effectively distinguished.Conclusion:After sulfur fumigation,the contents of ginsenoside Re and Rb1in Panacis Quinquefolii Radix decrease significantly,but the contents of 11 inorganic elements increase,suggesting that sulfur fumigation may have a great influence on the quality of Panacis Quinquefolii Radix.
作者 闫舒 肖世长 王为其 余阳 孙旭杰 王慧颖 侯磊 蒋俊 YAN Shu;XIAO Shi-chang;WANG Wei-qi;YU Yang;SUN Xu-jie;WANG Hui-ying;HOU Lei;JIANG Jun(Adverse Drug Reaction Monitoring Center,Zhenjiang Institute for Food and Drug Control,Zhenjiang 212000,China;School of Medicine,School of Pharmacy,Jiangsu University,Zhenjiang 212013,China;Integrated Technology Center,Zhenjiang Customs,Zhenjiang 212008,China)
出处 《中国实验方剂学杂志》 CAS CSCD 北大核心 2019年第23期116-121,共6页 Chinese Journal of Experimental Traditional Medical Formulae
基金 国家自然科学基金项目(81703773) 镇江市重点研发计划项目(SH2017054) 江苏省自然科学基金项目(BK20170560) 中国博士后科学基金特别资助项目(2018T110461)
关键词 硫磺熏蒸 西洋参 电感耦合等离子体发射光谱串联质谱 人参皂苷类 无机元素 主成分分析 微波消解法 sulfur fumigation Panacis Quinquefolii Radix inductively coupled plasma emission spectrometry tandem mass spectrometry ginsenosides inorganic elements principal component analysis microwave digestion method
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