基于成分特征的桑叶经霜质量标志物探索

Study on Frosting Quality Markers of Mori Folium Based on Component Characteristic

目的:分析识别桑叶经霜质量标志物(Q-marker),对桑叶是否经霜进行鉴别。方法:建立桑叶经霜前后HPLCDAD-MSn指纹图谱,标记共有峰,采用正交偏最小二乘判别分析(OPLS-DA)提取桑叶经霜后的特征成分,以含量变化较大的成分作为标志成分,较为稳定的成分作为内参成分,以标志成分与内参成分峰面积的相对比值为桑叶经霜成分特征,实现对桑叶经霜的鉴别,并通过MSn和对照品进行定性研究。结果:建立了桑叶经霜前后的指纹图谱,共标记了33个共有峰,通过OPLS-DA分析,峰1,23,14确定为标志成分,峰12确定为内参成分,通过MSn和对照品分别鉴定为枸橼酸衍生物,桑皮苷F,色氨酸和新绿原酸。桑叶经霜前后枸橼酸衍生物,桑皮苷F,色氨酸与新绿原酸峰面积的比值分别为0. 15±0. 054,1. 0±0. 48;0. 14±0. 073,0. 98±0. 48;0. 13±0. 088,0. 89±0. 49。结论:建立的桑叶指纹图谱中,枸橼酸衍生物,桑皮苷F,色氨酸与新绿原酸的峰面积比值作为桑叶经霜的成分特征具有专属性,可作为桑叶经霜Q-marker对桑叶是否经霜进行鉴别。该研究扩展了中药Q-marker专属性的内涵,可为桑叶的质量评价及成药性提供实验数据,并为类似研究提供参考。

Objective:To screen the frosting qualitymarkers(Q-marker)of Mori Foliumand identify Mori Folium after frost.Method:The HPLC-DAD-MSnfingerprints of Mori Folium before and after frost were established,the common peaks were markedand the characteristic components were selected by orthogonal partial least squares discrimination analysis(OPLS-DA).The components with a large content change were used as marker components,and the relatively stable component was used as an internal reference component.The ratio of the marker components to the peak area of the internal reference component was used as component characteristic to identify the frost of Mori Folium,andqualitative study of frosting markerswere performed by MSnand authentic standards.Result:The fingerprints were established and 33 common peaks were marked.Through OPLS-DA,peaks 1,23,14 were determined as marker components,peak 12 was determined as internal reference component.The frosting quality markers of Mori Folium were characterized as citric acid derivatives,saponin F,tryptophan and neochlorogenic acid by MSnand standards.The ratios of the peak areas of citric acid derivatives,saponin F,tryptophan and neochlorogenic acid before and after frost were 0.15±0.054,1.0±0.48;0.14±0.073,0.98±0.48,0.13±0.088,0.89±0.49,respectively.Conclusion:In the fingerprints established in this study,the peak area ratios of citric acid derivatives,saponin F,tryptophan to new chlorogenic acid had specificity,which can be used as frosting quality markers for identification of Mori Folium.This study expanded the connotation of the Q-marker specificity of traditional Chinese medicine.The results can provide experimental data for the quality evaluation and drug-forming properties of Mori Folium,and provide reference for similar research.