油菜籽蛋白质中硒含量及分布研究

Studies on the Content and Distribution of Selenium Protein in Rapeseed

采用补硒栽培的油菜籽经粉碎 ,脱脂 ,提取可溶性蛋白质 ,通过分级盐析 ,分成A、B、C、D、E 5组 ,用极谱法测定硒含量 ,探讨油菜籽中含硒蛋白质的分布 ,结果表明 :蛋白质结合硒是油菜富集硒的主要形式 ,油菜可溶性蛋白质中有多种硒蛋白质存在 ,在各盐析组分中均有含硒蛋白质存在 ,以D组硒含量最高 ,其分布次序为D >E >C >B >A。

The soluble protein,abstracted from rapeseed by adding selenium planting were classed to five groups of A?B?C?D and E through grading salting out.The Se protein in rapeseed were tested by polarograplug to study selemium distribution,the result showed that selenium that combines protein was major form in rapeseed used selenium. There were various Se protein in the soluble oilseed proteins. In the method of classed group, there was selenium protein in every group protein with the most content in D. The content of selenium in various groups was D>E>C>B>A.