一起由细菌混合感染引起的旅行团食物中毒案例分析

Food poisoning caused by Bacillus cereus and enterotoxin,Vibrio parahemolyticus in travel agency

目的快速、准确地检测引起群体性食物中毒的有毒物质及其含量,为食物中毒应急处置提供科学依据。方法根据食品安全事故流行病学调查技术指南及WS/T 82—1996、WS/T 81—1996诊断标准,采用常规方法及多重实时荧光PCR技术对送检的30份样品进行检测。结果分别在送检的1份呕吐物中检出蜡样芽胞杆菌肠毒素,3份腹泻物中检出副溶血性弧菌,2份餐余食物中检出蜡样芽胞杆菌,1份餐余食物中检出副溶血性弧菌。结论本次食源性疾病事件由蜡样芽胞杆菌及其肠毒素、副溶血性弧菌混合感染引起。

Objective To determine toxins in food poisoning and its contents fast and accurately,and to provide a scientific basis for emergency response of food poison. Methods According to the epidemiological investigation guide of food safety accidents and the diagnosis standard of WS / T 82-1996、WS / T 81-1996,routine methods and multiplex realtime PCR were used for 30 suspected samples involved vomits、diarrhea contents、food remainder by local CDC. Results Waxy bacillus enterotoxin was detected from vomits, and Bacillus cereus was also found in 2 food remainder, Vibrio parahaemolyticus was found in 3 diarrhea contents and 1 food remainder. Conclusion This food safety accident was caused by mixed infection with Bacillus cereus and Vibrio parahaemolyticus.