杭菊花乙醇提取物对大鼠生殖发育毒性研究

Reproductive toxicity pilot study of Chrysanthemum ethanol extract on rats

目的研究杭菊花乙醇提取物(CME)经过亲代、宫内、哺乳期和断乳后持续暴露对子代的影响。方法设对照组和CME低、中和高剂量组,CME掺入饲料,剂量分别为0、1、3、9 g/kg BW;参照OECD TG 443开展一代延长试验,每组孕鼠20只及其子鼠每组40只(雌雄各半),子鼠断乳后持续给予CME 13周,测定生长发育、生殖发育、血象、血生化指标,做组织病理学检查。结果 CME高、中剂量组子鼠睁眼时间明显迟于对照组;低剂量组子代雌性大鼠出生后(PND)56～112天体质量明显高于对照组,子代雌性大鼠CME高、低剂量组红细胞计数(RBC)和血红蛋白浓度(HGB)均降低,CME高剂量组谷丙转氨酶(ALT)升高,高、中剂量组谷草转氨酶(AST)、血磷(P)升高,CME各剂量组血糖(GLU)均降低、乳酸脱氢酶(LDH)均升高;子代雄性大鼠CME高、低剂量组血清钾(K^+)降低;子代雌雄大鼠CME高剂量组脾脏/体质量系数均高于对照组;以上均差异有统计学意义(F=2.818～42.75,P<0.05)。结论低剂量CME促进子代雌性大鼠体质量生长发育,降低子代雌性大鼠的血糖,影响RBC、HGB和LDH;高、中剂量CME影响子代大鼠发育和子代雌性大鼠的肝功能,高剂量CME影响子代雌雄大鼠脾脏/体质量系数。

Objective To study the effects of Chrysanthemum ethanol extract(CME)on rat offsprings with continuously exposure during parental,intrauterine,lactation and post-weaning.Methods CME was mixed into the feed to feed rats,which were divided into control,low,medium and high dose groups with dosage of 0,1,3,9 g/kg BW.Extended one-generation was carried out according to OECD TG 443 with 20 pregnant rats and 40 offspring in each group(half female and half male).CME was continuously given to offspring rats for 13 weeks after weaning.During the experiment,growth,reproductive development,hematology,biochemistry and histopathology were measured.Results The eyes opening time of the infant rat in the middle and high dose group of CME was significantly later than that of the control group.The weight of female offspring rats from postnatal day(PND)56-112 was significantly higher than that of the control group.In the high-dose group and low-dose group of female offspring rats,red blood cell(RBC)and hemoglobin(HGB)were decreased.In the high-dose group of female offspring rats,glutamic-pyruvic transaminase(ALT)increased.In the high-dose group of female rats,glutamic oxalacetic transaminase(AST)and serum phosphorus(P)increased.The glucose(GLU)decreased and the lactate dehydrogenase(LDH)increased,in CME treated female offspring group.The potassium(K^+)decreased in males offspring rats of high and low dose groups.The spleen coefficient in the high dose group was significantly higher than that in the control group.The above differences were statistically significant(F=2.818-42.75,P<0.05).Conclusion Low dosage CME promotes the body growth,the RBC,HGB and LDH were affected in female offspring rats;middle and high dosage CME affects development,liver function of infant rat,high dosage affects spleen coefficient in male and female offspring rats.