石家庄市零售鸡肉样品中弓形杆菌污染调查

Contamination of Arcobacter in retail chicken meat in Shijiazhuang City

目的调查石家庄市零售鸡肉中弓形杆菌的污染情况,为石家庄市弓形杆菌分布特征和防控提供基础数据支持。方法从石家庄市市内四区的菜市场、超市、连锁肉店随机购买零售鸡肉样品90份,应用驱动增强动力双孔滤膜法从样品中分离培养弓形杆菌,利用弓形杆菌多重聚合酶链式反应(PCR)检测及测序的方法对分离菌株进行种属鉴定,利用16S rDNA、rpoB基因测序的方法验证菌种鉴定结果。结果 90份鸡肉样品中,60份样品检出弓形杆菌86株,其中嗜低温弓形杆菌49株、布氏弓形杆菌37株,弓形杆菌检出率为66.67%(60/90)。60份阳性样品中,嗜低温弓形杆菌阳性样品45份(75.00%),布氏弓形杆菌阳性样品35份(58.33%)。嗜低温弓形杆菌和布氏弓形杆菌混合感染阳性样品20份,占比为22.22%(20/90)。不同采样点弓形杆菌检出率有较大差异。结论石家庄市零售鸡肉中弓形杆菌污染较重,嗜低温弓形杆菌污染率高于布氏弓形杆菌,两种弓形杆菌混合污染样品率较高。

Objective To investigate the contamination of Arcobacter in retail chicken meat in Shijiazhuang City,and provide basic data support for the distribution characteristics and prevention and control of Arcobacter in Shijiazhuang.Methods A total of 90 chicken meat samples were randomly collected from wholesale markets,supermarkets and butchers in Shijiazhuang.Enhanced membrane filter technique was used to isolate Arcobacter from the chicken meat samples,multiplex polymerase chain reaction(PCR)detection was used to identify Arcobacter and 16 S rRNA and rpoB genes of the Arcobacter isolates were sequenced and analyzed to confirm the detection result.Results Among the 90 samples,60 samples were Arcobacrer positive(66.67%),and 86 Arcobacter strains were isolated.Forty-nine of the eighty-six isolates were A.cryaerophilus and the rests were A.butzleri.Among the 60 Arcobacter positive samples,45 samples(75.00%)were A.cryaerophilus positive and 35 samples(58.33%)were A.butzleri positive.About 22.22%(20/90)samples were cocontaminated with A.cryaerophilus and A.butzleri.There were significant differences in the contamination rate of Arcobacter in different markets.Conclusion In Shijiazhuang City,the contamination of Arcobacter in retail chicken meat was serious,and the contamination rate of A.cryaerophilus was higher than A.butzleri in chicken meat samples.The contamination rate of A.cryaerophilus and A.butzleri were high.