利用改良的Chelex方法快速调查田间新蚜虫疠霉的流行

Using Modified Chelex Method for Rapid Epizootiological Investigation of Entomophthoralean Fungi(Pandora neoaphidis)

对昆虫病原体的直接取样、检测及定量化能够直接反映昆虫流行病学调查中的病原体丰度。本文以在田间蚜群中广泛流行的虫霉目真菌新蚜虫疠霉为研究对象,通过对不同来源的新蚜虫疠霉样品的DNA提取测试,建立Lysis提取结合Chelex-100方法的PCR检测技术体系,并以此方法为支撑进行了田间麦蚜新蚜虫疠霉流行病的调查验证。结果表明改良的Chelex方法不仅为昆虫病原真菌的田间流行学调查提供了一个快速、准确的评价方法,而且在其他真菌的核酸快速、痕量检测方面也可能具有重要的应用潜力。

Direct sampling, detection and quantification of entomopathogens can directly reflect the epizootiology of pest in abundance. Pandora neoaphidis, a widespread obligated-aphid pathogen, was investigated in aphid populations. A PCR technology system based on modified Lysis extraction associated with Chelex-100 was developed for epizootiological investigation of entomophthoralean fungi on wheat aphid. Results show that the developed method not only provide a rapid and accurate tool for epizootiological investigation of entomopathogenic fungus, but also have a potential application for rapid and tiny-nucleic acid detection in other fungus.