对华北大黑鳃金龟幼虫高毒力绿僵菌菌株的筛选及分子鉴定

Screening and Molecular Identification of Metarhizium Strains against Holotrichia oblita

绿僵菌Metarhizium anisopliae是一类对多种害虫具有高活性的病原真菌。本研究以华北大黑鳃金龟Holotrichia oblita 1龄幼虫为对象,从4株绿僵菌中筛选出1株毒力较高的菌株,命名为M7-9。采用浸渍法对华北大黑鳃金龟1龄幼虫进行毒力测定,结果表明,绿僵菌M7-9的致死率为96.67%,僵虫率为96.67%,致死中时为2.82 d;致死中浓度为1.29×106孢子/m L;绿僵菌M7-9 ITS序列分子鉴定表明该株绿僵菌为金龟子绿僵菌。

Metarhizium is a highly toxic pathogenic fungus against a variety of pests. A strain M7-9 showing high virulence to Holotrichia oblita 1st instar larvae was screened from four Metarhizium strains. Mortality rate and cadaver rate by the fungus were both 96.67%, LT50 and LC50 were 2.82 d and 1.29×106 spores/mL, respectively. ITS sequence analysis showed that the strain M7-9 is M. anisopliae.