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对华北大黑鳃金龟幼虫高毒力绿僵菌菌株的筛选及分子鉴定 被引量:6

Screening and Molecular Identification of Metarhizium Strains against Holotrichia oblita
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摘要 绿僵菌Metarhizium anisopliae是一类对多种害虫具有高活性的病原真菌。本研究以华北大黑鳃金龟Holotrichia oblita 1龄幼虫为对象,从4株绿僵菌中筛选出1株毒力较高的菌株,命名为M7-9。采用浸渍法对华北大黑鳃金龟1龄幼虫进行毒力测定,结果表明,绿僵菌M7-9的致死率为96.67%,僵虫率为96.67%,致死中时为2.82 d;致死中浓度为1.29×106孢子/m L;绿僵菌M7-9 ITS序列分子鉴定表明该株绿僵菌为金龟子绿僵菌。 Metarhizium is a highly toxic pathogenic fungus against a variety of pests. A strain M7-9 showing high virulence to Holotrichia oblita 1st instar larvae was screened from four Metarhizium strains. Mortality rate and cadaver rate by the fungus were both 96.67%, LT50 and LC50 were 2.82 d and 1.29×106 spores/mL, respectively. ITS sequence analysis showed that the strain M7-9 is M. anisopliae.
出处 《中国生物防治学报》 CSCD 北大核心 2014年第6期834-838,共5页 Chinese Journal of Biological Control
基金 现代农业产业技术体系(CARS-14) 国家"973"计划(2009CB118902) 国家自然科学基金(30971910)
关键词 金龟子绿僵菌 毒力测定 ITS序列分析 Metarhizium anisopliae virulence analysis of ITS sequence
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