肠道可培养共生菌对橘小实蝇不育雄虫生物学特征的影响

Impacts of Cultivable Gut Microbes on Biological Characteristics of Sterile Males of Bactrocera dorsalis(Hendel)

橘小实蝇是一种对果、蔬产业造成严重经济损失的害虫,应用昆虫不育技术(SIT)防治橘小实蝇是一种切实可行的科学手段,但辐照会使不育雄虫受到一定程度的损伤。本研究通过在饲料中添加抗生素对肠道灭菌处理来探究肠道可培养共生菌对橘小实蝇生物学及形态学参数的影响。结果表明,抗生素处理组在不育雄虫的蛹重、逆境存活率以及竞争交配力等均显著低于对照组。另外,抗生素处理组和对照组不育雄虫的存活率曲线图表明处理组不育雄虫的寿命时长显著低于对照组,利用Weibull分布拟合模型判断得知两组橘小实蝇存活率曲线均符合基本模型中的Ⅰ型。本研究为进一步利用肠道可培养共生菌来改善橘小实蝇不育雄虫的生理健康提供理论基础。

Bactrocera dorsalis(Hendel) is a notorious agricultural pest of fruit and vegetable industry. Sterile insect technique is widely used in control of B. dorsalis, but sterile males may be injured during radiation. In this study, the influence of cultivable gut microbes on biology and morphology of B. dorsalis was investigated through adding two antibiotics to the larval diet. The pupal weight, survival rate and mating competition of the antibioticstreated sterile males were significantly lower than the control sterile males. Further, although the survival curves of the antibiotics-treated and control sterile males fitted with pattern I of the basic Weibull distribution model, longevity of the antibiotics-treated sterile males was significantly shorter than that of the control. This result suggest that gut microbes can be used to reduce the irradiation-induced damage to Bactrocera dorsalis.