枯草芽胞杆菌Y13挥发性物质的分析及抑菌活性

Analysis of Volatile Compounds from Bacillus subtilis Y13 and Its Antimicrobial Activity

枯草芽胞杆菌Bacillus subtilis Y13是一株高效广谱的生防菌,对油茶主要病害具有较好的生防效果,试验表明其发酵产生的挥发性物质对油茶炭疽病菌有较好的抑制作用。对菌株Y13产生的挥发性物质进行定性定量分析,并测定其抑菌活性,为油茶病害无公害防治及药剂开发提供理论依据。采用顶空固相微萃取技术(HS-SPME)和气相色谱-质谱联用仪(GC-MS)收集并分析Y13挥发性物质成分,购买相应挥发性物质纯品,采用双皿倒扣法测定其抑菌活性。Y13挥发性物质对10种植物病原真菌抑菌率在19.2%~83.9%,其中挥发性物质对果生炭疽菌Colletotrichum fructicola的抑菌活性最强,抑菌率达到83.9%;检测其中10种挥发性化合物纯品对果生炭疽菌的抑菌活性发现,除了正十八烷外,其他9种挥发性化合物纯品均具有不同程度的抑菌活性;筛选出的4种高效抑菌挥发性化合物纯品对7种常见的油茶病原真菌的抑菌活性检测结果表明,4种挥发性化合物纯品(10μL/平板)对7种常见的油茶病原真菌均具有较好的抑菌活性,抑菌活性壬醛>苯甲醛>3-甲基-4-苯基吡唑>苯丙噻唑。Y13挥发性物质对油茶主要病害病原菌有很好的抑制作用,具有一定的研究意义和良好的开发价值。

Bacillus subtilis Y13 is a high-efficiency and broad-spectrum biocontrol strain.It has excellent biocontrol effect on the main diseases of Camellia oleifera.Experiments showed that the volatile substances produced by fermentation had a good inhibitory effect on anthracnose of Camellia oleifera.Volatile substances produced by strain Y13 were analyzed qualitatively and quantitatively,and their bacteriostatic activities were determined,which provided a theoretical basis for pollution-free control of Camellia oleifera diseases and pesticide development.Headspace solid-phase microextraction(HS-SPME)and gas chromatography-mass spectrometry(GC-MS)were used to collect and analyze the volatile components of Y13,purchasing pure volatile substances,and measuring its antifungus activity.The antifungus rate of Y13 volatiles to 10 plant pathogenic fungi was 19.2%-83.9%,among which volatile substances had the strongest antifungus activity against C.fructicola,and the inhibition rate reached 83.9%.The antifungus activity of 10 volatile compounds against C.fructicola was detected.Except octadecane,the other 9 volatile compounds had different degrees of antifungus activity.The four highly effective antifungus volatile compounds were selected.The antifungus activity of pure products against 7 common Camellia oleifera fungi showed that the four volatile compounds(10μL/plate)had good antifungus activity and antifungus activity against7 common Camellia oleifera fungi.The antifungus activity were ranked as Nonanal>Benzaldehyde>3-methyl-4-phenylpyrazole>Benzothiazole.Y13 volatile substances have a good inhibitory effect on the pathogens of Camellia oleifera,and have certain research significance and good development value.