对红细胞葡糖6-磷酸脱氢酶活性的几种筛选方法的评价

Evaluation of Several Methods for Screening Erythrocyte Glucose-6-phosphatase Dehydrogenase (G-6PD) Deficiency

红细胞葡糖6-磷酸脱氢酶(G-6PD)缺乏是多种溶血性疾患的重要病因之一。对人群进行G-6PD活性普查或对临床溶血病人进行过筛,都需要选择比较准确、快速、简便易行的方法。为此目的,目前已有多种方法。但这些方法又都必须与酶活性直接测定结果加以比较后,才能进行正确评价。对此,文献中尚未见有报道。我们用改良四氮唑蓝法、荧光斑点法、美蓝吸收法和高铁血红蛋白还原试验法检查了一批健康人和G-6PD缺乏者,并分别与同时进行的酶活性直接测定法(分光光度法)进行了比较。现将结果报道如下。

To detect erythrocyte G-6PD deficiency four common screen-methods, the methemoglobin reduction test(MHb-T), the methylene blue absorption test, the fluorescence spot test, and the modified nitroblue tetrazolium spot test(NBT-T), were compared with the enzyme quantitative assay.The results suggest that the fluorescence spot test and modified NBT-test agrees well with the G-6PD activity determinations, and are more favorable due to their high-speed, simplicity and specificity.The MHb-T and methylene blue absorption test were also evaluated.