鼻咽癌免疫抑制因子对NK细胞功能的影响

A Study on the Immunological Inhibitors in Nasopharyngcal Cancer-The NPC Immunological Inhibitors Factor Effect of NK Cells Function

T细胞在免疫监视和抗肿瘤中的重要作用已有较深入的研究。近几年来又发现天然杀伤细胞(NaturaI Killer CeII,NK细胞)不依赖抗体或补体,也不需预先致敏就能溶解多种的肿瘤细胞。因此,人们提出了NK细胞在抗肿瘤中的独特作用——免疫监视功能。动物实验发现肿瘤生长旺盛时,宿主NK细胞活性降低。在NK细胞活性高的受体内接种肿瘤,肿瘤的生长情况较在NK细胞活性低的受体内差。在人类的一些癌肿患者NK细胞活性也是低下的。提示了NK细胞的功能与肿瘤发生发展的关系。

Previous research work of authors had shown that the Inhibitory effect of the Cancerous tissue extract of patients with NPC to peripheral lymphocy-tic transformation, However,what is effect to the NK Cell treated with inhibitory factors is not yet solved.This report showed the result effect of NK Cells mediated cytolysis that against K562 Cells by 51Cr release assay.The cytolysis rate of 28 normal subjects was 58.42 ± 13%, When the NPC tissue extract added to lymphocyte suspension, the cytolysis rate was decreased from 58.42 ± 13% to 15.9 ± 7.2%.After the NPC tissue extract wac filtrated by sephadex G200, obtaining the (IV) fraction protein, the cytolysis rate was 40.3 ± 12% shown lower ( P< 0.05). However, using tonsis tissue extract, the cytolysis rate was 54,4± 7.5%, (P>0.05).These results was shown that the NPC immunological inhibitore factor may inhibit both T Cell and NK Cell.