艾美尔球虫子孢子体外刺激鸡异嗜白细胞和巨噬细胞炎性细胞因子表达水平

Expression of inflammatory cytokines in heterophils and macrophages stimulated with Eimeria tenella in vitro

以实时荧光定量PCR方法检测了艾美尔球虫(Eimeria tenella)子孢子体外刺激鸡异嗜白细胞和巨噬细胞炎性细胞因子IFN-γ、IL-6、IL-10和IL-12mRNA表达水平。结果发现,与不刺激对照组相比,活的E.tenella子孢子刺激异嗜白细胞(4h)和巨噬细胞IL-6mRNA表达水平显著增加(P<0.05)。灭活的E.tenella子孢子刺激异嗜白细胞和巨噬细胞IFN-γ、IL-6、IL-10和IL-12mRNA表达水平均高于活的E.tenella子孢子,差异显著(P<0.05)。与不刺激对照组相比,灭活的E.tenella子孢子刺激异嗜白细胞IL-6、IL-10、IL-12(2h)和IFN-γ(4h)mRNA表达水平均显著增加(P<0.05),灭活的E.tenella子孢子刺激巨噬细胞IL-6、IL-12和IFN-γmRNA表达水平显著上调(P<0.05)。表明,鸡异嗜白细胞和巨噬细胞受到E.tenella子孢子刺激后炎性细胞因子的显著上调可能与机体清除球虫感染有关。

The expression of inflammatory cytokines in heterophils and macrophages stimulated with Eimeria tenella in vitro were detected in the experiment.The results showed that the expression of IL-6in heterophils(4h)and macrophages stimulated by live E.tenella sporozoites were increased significantly than that unstimulated cells(P<0.05).The expression of IFN-γ,IL-6,IL-10 and IL-12 by stimulated heat-killed E.tenella sporozoites was higher than live sporozoites(P>0.05),the expression of IL-6,IL-10,IL-12(2h),IFN-γ(4h)in heterophils,and IL-6,IL-10,IL-12 in macrophages stimulated with heat-killed E.tenella sporozoites were up-regulated significantly than unstimulated cells(P<0.05).The results suggest that the significant up-regulation of inflammatory cytokines in heterophils and macrophages may associate with the eliminating of E.tenella.