2013年苏皖地区鹅细小病毒的分离鉴定及其VP3基因序列分析

Isolation and identification of goose parvovirus and sequence analysis for VP3 gene

鹅细小病毒(GPV)主要引起雏鹅和雏番鸭的高死亡率。本研究对2013年采集的苏皖地区疑似GPV感染的病鹅组织,通过鹅胚病毒分离以及PCR扩增,共分离鉴定出11株GPV病毒。这11株GPV病毒的鹅胚致死时间为43~159h。与NCBI中检索到的21个VP3进行的序列分析发现,这些GPV的VP3氨基酸同源性为97.2%~100%。进化树分析表明,新分离的GPV VP3与分离于1982年的台湾GPV毒株82-0308最接近。与其他分离株VP3比较,这11株中有3株VP3中存在N35D、V261L、Y293H共突变现象。这些苏皖地区GPV毒株的分离、鉴定以及序列特征,对进一步探究苏皖地区GPV病毒的分子演化特征以及流行病学提供了材料。

Goose parvovirus(GPV)causes high mortality in goslings and Muscovy ducklings.In this study,11 isolates of GPVs from Suwan region were obtained by inoculating goose embryo eggs with tissue supertanant of sick goslings suspected with GPV infection and identified by PCR and sequencing.The lethal times for the goose embryo eggs inoculated with these isolates were from 43 to 159h.Sequence analysis showed that the amino acid sequence of VP3 among the 11 isolates had97.2%-100%identity with the 21 isolates from NCBI.Further phylogenic tree assay suggested that 11 isolates were genetic closely to the Taiwan Residents isolate 82-0308.In comparison with isolates published in NCBI,three co-evolution/mutation sites,N35 D,V261L,Y293 H,were found in 3of these 11 isolates.These GPV isolates and findings provide insights into molecular characteristics and epidemic of GPV in Suwan region.