河北省PRRSV流行毒株的分离鉴定

Isolation and identification of PRRSV epidemic strains in Hebei Province

为了全面调查猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)在河北省的流行情况,本研究共收集河北省833份病死猪临床样品,利用RT-PCR方法对其进行PRRSV检测,共检出419份阳性病料,阳性率高达50.3%。其中NADC30-like PRRSV阳性率为49.1%;HP-PRRSV阳性率为3.1%;经典株PRRSV没有检出;HP-PRRSV和NADC30-like PRRSV共感染样品为16份,共感染率为1.9%。此外,本研究成功地分离到3株NADC30-like毒株,并测通其全基因组序列,与国内外流行毒株进行序列比对和基因重组分析发现,这3个毒株中的QHD1株为重组毒株,亲本可能为PRRSV NADC30毒株和疫苗株RespPRRS MLV,2处重组分别位于7 913～8 015nt和12 780～13 158nt处。本研究为河北省PRRSV的深入研究和防控奠定了基础。

In order to investigate the epidemic situation of porcine reproductive and respiratory syndrome virus(PRRSV)in Hebei Province,833clinical samples of sick and dead pigs were collected and detected PRRSV by RT-PCR in this study.The results revealed that a total of 419positive samples were detected,with a positive rate of 50.3%.Thereinto,the positive rate of NADC30-like PRRSV was 49.1%,the positive rate of HP-PRRSV was 3.1%,and the classic strain PRRSV was not detected.The co-infection samples of HP-PRRSV and PRRSV NADC30-like strains were 16 and the co-infection rate was 1.9%.In addition,three NADC30-like strains were successfully isolated and their whole genome were sequenced.Compared with PRRSV epidemic strains through the sequence alignment and gene recombination analysis,the QHD1isolate was a recombinant strain,and the parent strains may be the PRRSV NADC30strain and the vaccine strain RespPRRS MLV.The two recombination regions were located at 7 913-8 015nt and 12 780-13 158nt,respectively.The research laid the groundwork for the further study on the prevention and control of PRRSV in Hebei Province.