大肠杆菌对奶牛子宫内膜组织合成分泌IL-1β、IL-6和PGE2的影响

Effects of Escherichia coli on synthesis and secretion of IL-1β,IL-6 and PGE2 in dairy cow endometrium explants in vitro

基于奶牛子宫内膜组织体外培养方法,分别采用浓度为1×105,1×106 CFU/mL的致病性大肠杆菌处理体外培养的子宫内膜组织12,24 h后用HE染色方法评价组织损伤情况,ELISA法检测组织培养液上清中IL-1β,IL-6和PGE2的分泌变化,RT-qPCR法检测IL-1β和IL-6基因表达变化。结果显示:与对照组相比,大肠杆菌分别处理12,24 h后奶牛子宫内膜组织中子宫内膜上皮细胞和腺上皮细胞脱落、坏死、崩解,并且显著提高奶牛子宫内膜组织IL-1β,IL-6和PGE2的分泌及IL-1β和IL-6的mRNA的表达量。结果表明:成功建立了体外大肠杆菌型奶牛子宫内膜炎模型,且IL-1β,IL-6和PGE2在大肠杆菌引起的奶牛子宫内膜组织炎症反应中发挥着重要的调控作用。

Endometrial explants in vitro culture,were stimulated by endometrial pathogenic E.coli(1×105,1×106 CFU/mL)for 12,24 h,followed by the detection of endometrial explants damage by HE,the section of IL-1β,IL-6 and PGE2 in the endometrial explants supernatant by ELISA and the differential expression of IL-1βand IL-6 by RT-PCR.The results showed that the histological sections of E.coli induced endometrium indicated that the endometrial glands were not clearly visible,and endometrial epithelial cells and glandular epithelial cells had disappeared after treatment of E.coli for 12,24 h,and the IL-1β,IL-6 and PGE2 section and IL-1β,IL-6 mRNA expression were significantly up-regulated compared with control group.The results indicated that the in vitro cultivation method of Escherichia coli induced bovine endometrial tissue was successfully established,and IL-1β,IL-6 and PGE2 may play an important role in the inflammatory response of bovine endometrium induce by E.coli.